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  In situ opening/closing of OmpG from E. coli and the splitting of β-sheet signals in ATR–FTIR spectroscopy

Korkmaz, F., Köster, S., Yildiz, Ö., & Mäntele, W. (2012). In situ opening/closing of OmpG from E. coli and the splitting of β-sheet signals in ATR–FTIR spectroscopy. Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy, 91, 395-401. doi:10.1016/j.saa.2012.01.025.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-D5AF-F Versions-Permalink: https://hdl.handle.net/21.11116/0000-000C-D1B8-8
Genre: Zeitschriftenartikel

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 Urheber:
Korkmaz, Filiz1, Autor
Köster, Stefan2, Autor           
Yildiz, Özkan2, Autor                 
Mäntele, Werner1, Autor
Affiliations:
1External Organizations, ou_persistent22              
2Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              

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Schlagwörter: β-Barrel membrane protein; Secondary structure determination; FT-IR spectroscopy; Amide-I band analysis; Curve-fitting; Difference spectrum
 Zusammenfassung: The pH dependent opening and closure of Escherichia coli OmpG is driven by the formation and breaking of hydrogen bridges in β-strands S11-S13. We have investigated the in situ secondary structural changes of OmpG with ATR-FTIR difference spectroscopy in order to detect the signals associated with the newly established interactions. Curve-fitting of OmpG in two pH conditions revealed the splitting and shifting of β-sheet signals upon opening of the channel. Besides secondary structure changes, there are also amino acid side chain signals that play active role in opening/closing of the channel. An interaction among positively charged arginines and negatively charged aspartic and glutamic acid residues is suggested upon closure of the channel while this interaction is abolished when the channel opens at higher pH.

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Sprache(n): eng - English
 Datum: 2012-02-152012-06
 Publikationsstatus: Erschienen
 Seiten: 7
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: eDoc: 630201
DOI: 10.1016/j.saa.2012.01.025
 Art des Abschluß: -

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Titel: Spectrochimica Acta, Part A: Molecular and Biomolecular Spectroscopy
  Andere : Spectrochim. Acta Part A
  Andere : Spectrochim. Acta, Part A
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Kidlington, Oxford, U.K. : Elsevier
Seiten: - Band / Heft: 91 Artikelnummer: - Start- / Endseite: 395 - 401 Identifikator: ISSN: 1386-1425
CoNE: https://pure.mpg.de/cone/journals/resource/954926979061