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  Structure of the c10 ring of the yeast mitochondrial ATP synthase in the open conformation

Symersky, J., Pagadala, V., Osowski, D., Krah, A., Meier, T., Faraldo-Gómez, J. D., et al. (2012). Structure of the c10 ring of the yeast mitochondrial ATP synthase in the open conformation. Nature Structural and Molecular Biology, 19, 485-491. doi:10.1038/nsmb.2284.

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 Creators:
Symersky, Jindrich1, Author
Pagadala, Vijayakanth1, Author
Osowski, Daniel1, Author
Krah, Alexander2, Author           
Meier, Thomas3, Author           
Faraldo-Gómez, José D.2, Author           
Mueller, David M.1, Author
Affiliations:
1External Organizations, ou_persistent22              
2Max Planck Research Group of Theoretical Molecular Biophysics, Max Planck Institute of Biophysics, Max Planck Society, ou_2068295              
3Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              

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 Abstract: The proton pore of the F(1)F(o) ATP synthase consists of a ring of c subunits, which rotates, driven by downhill proton diffusion across the membrane. An essential carboxylate side chain in each subunit provides a proton-binding site. In all the structures of c-rings reported to date, these sites are in a closed, ion-locked state. Structures are here presented of the c(10) ring from Saccharomyces cerevisiae determined at pH 8.3, 6.1 and 5.5, at resolutions of 2.0 Å, 2.5 Å and 2.0 Å, respectively. The overall structure of this mitochondrial c-ring is similar to known homologs, except that the essential carboxylate, Glu59, adopts an open extended conformation. Molecular dynamics simulations reveal that opening of the essential carboxylate is a consequence of the amphiphilic nature of the crystallization buffer. We propose that this new structure represents the functionally open form of the c subunit, which facilitates proton loading and release.

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Language(s): eng - English
 Dates: 2012-04-152012-05
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 630894
DOI: 10.1038/nsmb.2284
 Degree: -

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Title: Nature Structural and Molecular Biology
  Other : Nature Struct Biol
Source Genre: Journal
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Publ. Info: New York, NY : Nature Pub. Group
Pages: - Volume / Issue: 19 Sequence Number: - Start / End Page: 485 - 491 Identifier: ISSN: 1545-9993
CoNE: https://pure.mpg.de/cone/journals/resource/954925603763