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  Ultra light-sensitive and fast neuronal activation with the Ca2+-permeable channelrhodopsin CatCh

Kleinlogel, S., Feldbauer, K., Dempski, R. E., Fotis, H., Wood, P. G., Bamann, C., et al. (2011). Ultra light-sensitive and fast neuronal activation with the Ca2+-permeable channelrhodopsin CatCh. Nature Neuroscience, 14(4), 513-518. doi:10.1038/nn.2776.

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 Creators:
Kleinlogel, Sonja1, Author              
Feldbauer, Katrin1, Author              
Dempski, Robert E.1, Author              
Fotis, Heike1, Author              
Wood, Phillip G.1, Author              
Bamann, Christian1, Author              
Bamberg, Ernst1, 2, Author              
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              
2Chemical and Pharmaceutical Sciences Department, Johann Wolfgang Goethe-University, Frankfurt am Main, Germany, ou_persistent22              

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 Abstract: The light-gated cation channel channelrhodopsin-2 (ChR2) has rapidly become an important tool in neuroscience, and its use is being considered in therapeutic interventions. Although wild-type and known variant ChR2s are able to drive light-activated spike trains, their use in potential clinical applications is limited by either low light sensitivity or slow channel kinetics. We present a new variant, calcium translocating channelrhodopsin (CatCh), which mediates an accelerated response time and a voltage response that is ~70-fold more light sensitive than that of wild-type ChR2. CatCh’s superior properties stem from its enhanced Ca2+ permeability. An increase in [Ca2+]i elevates the internal surface potential, facilitating activation of voltage-gated Na+ channels and indirectly increasing light sensitivity. Repolarization following light-stimulation is markedly accelerated by Ca2+-dependent BK channel activation. Our results demonstrate a previously unknown principle: shifting permeability from monovalent to divalent cations to increase sensitivity without compromising fast kinetics of neuronal activation. This paves the way for clinical use of light-gated channels.

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Language(s): eng - English
 Dates: 2010-08-192011-02-022011-03-132011-04-01
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nn.2776
PMID: 21399632
 Degree: -

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Title: Nature Neuroscience
  Other : Nat. Neurosci.
Source Genre: Journal
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Publ. Info: New York, NY : Nature America Inc.
Pages: - Volume / Issue: 14 (4) Sequence Number: - Start / End Page: 513 - 518 Identifier: ISSN: 1097-6256
CoNE: https://pure.mpg.de/cone/journals/resource/954925610931