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  Robust Electrophysiological Assays using Solid Supported Membranes: the Organic Cation Transporter OCT2

Gaiko, O., Janausch, I., Geibel, S., Vollert, H., Arndt, P., Gonski, S., et al. (2011). Robust Electrophysiological Assays using Solid Supported Membranes: the Organic Cation Transporter OCT2. Australian Journal of Chemistry, 64(1), 31-35. doi:10.1071/CH10322.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-D61F-D Versions-Permalink: https://hdl.handle.net/21.11116/0000-000A-764E-B
Genre: Zeitschriftenartikel

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 Urheber:
Gaiko, Olga1, Autor           
Janausch, Ingo1, Autor           
Geibel, Sven2, Autor
Vollert, Henning2, Autor
Arndt, Petra2, Autor
Gonski, Sigrid2, Autor
Fendler, Klaus1, Autor           
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              
2Sanovi-Aventis Germany GmbH, Industriepark Höchst, 65926 Frankfurt, Germany, ou_persistent22              

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Schlagwörter: -
 Zusammenfassung: An electrophysiological assay platform based on solid supported membranes (SSM) for the organic cation transporter (OCT) is presented. Stable Chinese hamster ovary (CHO) cell lines overexpressing the human (hOCT2) and rat transporters (rOCT2) were generated and validated. Membrane preparations from the cell lines were investigated using SSM-based electrophysiology. Baculovirus transfected insect cells (HighFive and Mimic Sf9) were also tested with the same assay but yielded less than optimal results. The assays were validated by the determination of substrate affinities and inhibition by standard inhibitors. The study demonstrates the suitability of the SSM-based electrophysiological OCT assay for rapid and automatic screening of drug candidates.

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Sprache(n): eng - English
 Datum: 2010-08-312010-10-122011-01-14
 Publikationsstatus: Erschienen
 Seiten: 5
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1071/CH10322
 Art des Abschluß: -

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Titel: Australian Journal of Chemistry
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Collingwood, Victoria, Australia : CSIRO Pub.
Seiten: - Band / Heft: 64 (1) Artikelnummer: - Start- / Endseite: 31 - 35 Identifikator: ISSN: 0004-9425
CoNE: https://pure.mpg.de/cone/journals/resource/954925383068