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  Energy-filtered transmission electron microscopy of biological samples on highly transparent carbon nanomembranes

Rhinow, D., Büenfeld, M., Weber, N.-E., Beyer, A., Gölzhäuser, A., Kühlbrandt, W., et al. (2011). Energy-filtered transmission electron microscopy of biological samples on highly transparent carbon nanomembranes. Ultramicroscopy, 111(5), 342-349. doi:10.1016/j.ultramic.2011.01.028.

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 Creators:
Rhinow, Daniel1, Author           
Büenfeld, Matthias2, Author
Weber, Nils-Eike2, Author
Beyer, André2, Author
Gölzhäuser, Armin2, Author
Kühlbrandt, Werner1, Author                 
Hampp, Norbert3, Author
Turchanin, Andrey2, Author
Affiliations:
1Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              
2University of Bielefeld, Department of Physics, 33615 Bielefeld, Germany, ou_persistent22              
3University of Marburg, Department of Chemistry, 35032 Marburg, Germany, ou_persistent22              

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Free keywords: Graphene; EELS; EFTEM; CryoEM; Support film; Nanomembrane
 Abstract: Ultrathin carbon nanomembranes (CNM) comprising crosslinked biphenyl precursors have been tested as support films for energy-filtered transmission electron microscopy (EFTEM) of biological specimens. Due to their high transparency CNM are ideal substrates for electron energy loss spectroscopy (EELS) and electron spectroscopic imaging (ESI) of stained and unstained biological samples. Virtually background-free elemental maps of tobacco mosaic virus (TMV) and ferritin have been obtained from samples supported by ∼1 nm thin CNM. Furthermore, we have tested conductive carbon nanomembranes (cCNM) comprising nanocrystalline graphene, obtained by thermal treatment of CNM, as supports for cryoEM of ice-embedded biological samples. We imaged ice-embedded TMV on cCNM and compared the results with images of ice-embedded TMV on conventional carbon film (CC), thus analyzing the gain in contrast for TMV on cCNM in a quantitative manner. In addition we have developed a method for the preparation of vitrified specimens, suspended over the holes of a conventional holey carbon film, while backed by ultrathin cCNM.

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Language(s): eng - English
 Dates: 2011-01-152010-09-072011-01-212011-02-012011-04-01
 Publication Status: Issued
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.ultramic.2011.01.028
PMID: 21329648
 Degree: -

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Title: Ultramicroscopy
  Abbreviation : Ultramicroscopy
Source Genre: Journal
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Publ. Info: Amsterdam : North-Holland
Pages: - Volume / Issue: 111 (5) Sequence Number: - Start / End Page: 342 - 349 Identifier: ISSN: 0304-3991
CoNE: https://pure.mpg.de/cone/journals/resource/954925512451