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  Projection Structure of Channelrhodopsin-2 at 6 Å Resolution by Electron Crystallography

Müller, M., Bamann, C., Bamberg, E., & Kühlbrandt, W. (2011). Projection Structure of Channelrhodopsin-2 at 6 Å Resolution by Electron Crystallography. Journal of Molecular Biology, 414(1), 86-95. doi:10.1016/j.jmb.2011.09.049.

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 Creators:
Müller, Maria1, Author           
Bamann, Christian2, Author           
Bamberg, Ernst2, Author           
Kühlbrandt, Werner1, Author                 
Affiliations:
1Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              
2Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              

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Free keywords: microbial rhodopsin; channelrhodopsin; light-gated ion channel; two-dimensional crystallography; electron cryo-microscopy
 Abstract: Channelrhodopsin-2 (ChR2) is the prototype of a new class of light-gated ion channels that is finding widespread applications in optogenetics and biomedical research. We present a 6-Å projection map of ChR2, obtained by cryo-electron microscopy of two-dimensional crystals grown from pure, heterologously expressed protein. The map shows that ChR2 is the same dimer with non-crystallographic 2-fold symmetry in three different membrane crystals. This is consistent with biochemical analysis, which shows a stable dimer in detergent solution. Comparison to the projection map to bacteriorhodopsin indicates a similar structure of seven transmembrane alpha helices. Based on the projection map and sequence alignments, we built a homology model of ChR2 that potentially accounts for light-induced channel gating. Although a monomeric channel is not ruled out, comparison to other membrane channels and transporters suggests that the ChR2 channel is located at the dimer interface on the 2-fold axis, lined by transmembrane helices 3 and 4.

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Language(s): eng - English
 Dates: 2011-09-272011-07-292011-09-292011-10-052011-11-18
 Publication Status: Issued
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.jmb.2011.09.049
PMID: 22001017
 Degree: -

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Title: Journal of Molecular Biology
  Other : JMB
  Abbreviation : J. Mol. Biol.
Source Genre: Journal
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Publ. Info: Elsevier
Pages: - Volume / Issue: 414 (1) Sequence Number: - Start / End Page: 86 - 95 Identifier: ISSN: 0022-2836
CoNE: https://pure.mpg.de/cone/journals/resource/0022-2836