Single-walled carbon nanotubes and nanocrystalline graphene reduce beam-induced 
movements in high-resolution electron cryo-microscopy of ice-embedded 
biological samples

Rhinow, Daniel; Weber, Niels-Eike; Turchanin, Andrey; Gölzhäuser, Armin; Kühlbrandt, Werner

doi:10.1063/1.3645010

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Single-walled carbon nanotubes and nanocrystalline graphene reduce beam-induced movements in high-resolution electron cryo-microscopy of ice-embedded biological samples

Rhinow, D., Weber, N.-E., Turchanin, A., Gölzhäuser, A., & Kühlbrandt, W. (2011). Single-walled carbon nanotubes and nanocrystalline graphene reduce beam-induced movements in high-resolution electron cryo-microscopy of ice-embedded biological samples. Applied Physics Letters, 99(3): 133701. doi:10.1063/1.3645010.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-D65A-7 Version Permalink: https://hdl.handle.net/21.11116/0000-000B-A107-7

Genre: Journal Article

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Creators:
Rhinow, Daniel¹, Author
Weber, Niels-Eike², Author
Turchanin, Andrey², Author
Gölzhäuser, Armin², Author
Kühlbrandt, Werner¹, Author

Affiliations:
1Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291
2University of Bielefeld, Department of Physics, 33615 Bielefeld, Germany, ou_persistent22

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Free keywords: biological specimen preparation; carbon nanotubes; doping; electron microscopy; graphene; nanobiotechnology; self-assembly; suspensions; thin films

Abstract: For single particle electron cryo-microscopy (cryoEM), contrast loss due to beam-induced charging and specimen movement is a serious problem, as the thin films of vitreous ice spanning the holes of a holey carbon film are particularly susceptible to beam-induced movement. We demonstrate that the problem is at least partially solved by carbon nanotechnology. Doping ice-embedded samples with single-walled carbon nanotubes (SWNT) in aqueous suspension or adding nanocrystalline graphene supports, obtained by thermal conversion of cross-linked self-assembled biphenyl precursors, significantly reduces contrast loss in high-resolution cryoEM due to the excellent electrical and mechanical properties of SWNTs and graphene

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Language(s): eng - English

Dates: Submitted: 2011-07-18Accepted: 2011-09-09Published Online: 2011-09-27

Publication Status: Published online

Pages: 3

Publishing info: -

Table of Contents: -

Rev. Type: Peer

Identifiers: DOI: 10.1063/1.3645010

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Title: Applied Physics Letters

Abbreviation : Appl. Phys. Lett.

Source Genre: Journal

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Publ. Info: Melville, NY : American Institute of Physics

Pages: - Volume / Issue: 99 (3) Sequence Number: 133701 Start / End Page: - Identifier: ISSN: 0003-6951
CoNE: https://pure.mpg.de/cone/journals/resource/954922836223