English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Effects of α-Asarone on the Glutamate Transporter EAAC1 in Xenopus Oocytes

Gu, Q., Du, H., Ma, C., Fotis, H., Wu, B., Huang, C., et al. (2010). Effects of α-Asarone on the Glutamate Transporter EAAC1 in Xenopus Oocytes. Planta Medica, 75(1), 595-598. doi:10.1055/s-0029-1240613.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Gu, Quanbao1, Author
Du, Huiming1, Author
Ma, Chunhui2, Author
Fotis, Heike3, Author           
Wu, Bin2, Author
Huang, Chenggang2, Author
Schwarz, Wolfgang1, 3, Author           
Affiliations:
1Shanghai Research Center for Acupuncture and Meridians, Shanghai, China, ou_persistent22              
2Shanghai Institute of Materia Medica, Chinese Academy of Sciences, Shanghai, China, ou_persistent22              
3Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              

Content

show
hide
Free keywords: Acorus tatarinowi; Acoraceae; α-asarone; neurotransmitter transporter; glutamate; Xenopus oocytes; voltage clamp
 Abstract: The major excitatory neurotransmitter transporter EAAC1 in the mammalian central nervous system is considered a possible target for Chinese herbal medicine. Extracts of Acorus tatarinowii (Schott) were tested for their effects on EAAC1 activity. Xenopus oocytes with heterologously expressed EAAC1 were used as the model system. Rate of glutamate uptake was determined by means of the isotopic tracer technique. Glutamate-induced current was recorded under a two-electrode voltage clamp. As a highly effective component, α-asarone was identified. The rate of glutamate uptake was stimulated by 200 µM of α-asarone by about 15 %. In contrast, the same concentration reduced the EAAC1-mediated current by about 35 % at a holding potential of − 60 mV; half maximum inhibition was obtained at about 60 µM. Our experimental data suggest that both stimulation of glutamate uptake and inhibition of EAAC1-mediated current by α-asarone could contribute to reduced excitatory activity.

Details

show
hide
Language(s): eng - English
 Dates: 2009-10-152009-09-172009-10-242009-11-202010-04-01
 Publication Status: Issued
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1055/s-0029-1240613
PMID: 19937551
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Planta Medica
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Stuttgart : Georg Thieme Verlag.
Pages: - Volume / Issue: 75 (1) Sequence Number: - Start / End Page: 595 - 598 Identifier: ISSN: 0032-0943
CoNE: https://pure.mpg.de/cone/journals/resource/954925434428