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  On the regulation of photosynthesis by excitonic interactions between carotenoids and chlorophylls

Bode, S., Quentmeier, C. C., Liao, P.-N., Hafi, N., Barros, T., Wilk, L., et al. (2009). On the regulation of photosynthesis by excitonic interactions between carotenoids and chlorophylls. Proceedings of the National Academy of Sciences of the United States of America, 106(30), 12311-12316. doi:10.1073/pnas.0903536106.

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Bode, Stefan1, Author
Quentmeier, Claudia C.1, Author
Liao, Pen-Nan1, Author
Hafi, Nour1, Author
Barros, Tiago2, Author           
Wilk, Laura2, Author           
Bittner, Florian3, Author
Walla, Peter J.1, 4, Author
1Technische Universität Braunschweig, Institute for Physical and Theoretical Chemistry, Department for Biophysical Chemistry, 38106 Braunschweig, Germany, ou_persistent22              
2Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              
3Technische Universität Braunschweig, Department of Plant Biology, 38106 Braunschweig, Germany, ou_persistent22              
4Max Planck Institute for Biophysical Chemistry, Department of Spectroscopy and Photochemical Kinetics, Am Fassberg 11, 37077 Göttingen, Germany, ou_persistent22              


Free keywords: Arabidopsis; LHCII; NPQ; two-photon excitation
 Abstract: Selective 2-photon excitation (TPE) of carotenoid dark states, Car S<sub>1</sub>, shows that in the major light-harvesting complex of photosystem II (LHCII), the extent of electronic interactions between carotenoid dark states (Car S<sub>1</sub>) and chlorophyll (Chl) states, <sub>φCoupling</sub><sup>Car S<sub>1</sub>−Chl</sup>, correlates linearly with chlorophyll fluorescence quenching under different experimental conditions. Simultaneously, a linear correlation between both Chl fluorescence quenching and <sub>φCoupling</sub><sup>Car S<sub>1</sub>−Chl</sup> with the intensity of red-shifted bands in the Chl Qsub>y</sub> and carotenoid absorption was also observed. These results suggest quenching excitonic Car S1−Chl states as origin for the observed effects. Furthermore, real time measurements of the light-dependent down- and up-regulation of the photosynthetic activity and <sub>φCoupling</sub><sup>Car S<sub>1</sub>−Chl</sup> in wild-type and mutant (npq1, npq2, npq4, lut2 and WT+PsbS) Arabidopsis thaliana plants reveal that also in vivo the quenching parameter NPQ correlates always linearly with the extent of electronic Car S1–Chl interactions in any adaptation status. Our in vivo measurements with Arabidopsis variants show that during high light illumination, φCouplingCar S1−Chl depends on the presence of PsbS and zeaxanthin (Zea) in an almost identical way as NPQ. In summary, these results provide clear evidence for a very close link between electronic Car S1–Chl interactions and the regulation of photosynthesis. These findings support a photophysical mechanism in which short-living, low excitonic carotenoid–chlorophyll states serve as traps and dissipation valves for excess excitation energy.


Language(s): eng - English
 Dates: 2009-03-312009-06-052009-07-28
 Publication Status: Issued
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1073/pnas.0903536106
PMID: 19617542
PMC: PMC2714278
 Degree: -



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Title: Proceedings of the National Academy of Sciences of the United States of America
  Other : PNAS
  Other : Proceedings of the National Academy of Sciences of the USA
  Abbreviation : Proc. Natl. Acad. Sci. U. S. A.
Source Genre: Journal
Publ. Info: Washington, D.C. : National Academy of Sciences
Pages: - Volume / Issue: 106 (30) Sequence Number: - Start / End Page: 12311 - 12316 Identifier: ISSN: 0027-8424
CoNE: https://pure.mpg.de/cone/journals/resource/954925427230