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  From protons to OXPHOS supercomplexes and Alzheimer's disease: Structure–dynamics–function relationships of energy-transducing membranes

Seelert, H., Dani, D. N., Dante, S., Hauß, T., Krause, F., Schäfer, E., et al. (2009). From protons to OXPHOS supercomplexes and Alzheimer's disease: Structure–dynamics–function relationships of energy-transducing membranes. Biochimica et Biophysica Acta, Bioenergetics, 1787(6), 657-671. doi:10.1016/j.bbabio.2009.02.028.

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 Creators:
Seelert, Holger1, Author
Dani, Diksha Narhar1, Author
Dante, Silvia2, Author
Hauß, Thomas1, 2, Author
Krause, Frank1, Author
Schäfer, Eva1, Author
Frenzel, Monika1, Author
Poetsch, Ansgar1, Author
Rexroth, Sascha1, Author
Schwaßmann, Helena J.1, Author
Suhai, Tina1, Author
Vonck, Janet3, Author                 
Dencher, Norbert A.1, 2, Author
Affiliations:
1Department of Chemistry, Technische Universität Darmstadt, Petersenstrasse 22, D-64287 Darmstadt, Germany, ou_persistent22              
2Helmholtz-Zentrum Berlin für Materialien und Energie, Lise-Meitner-Campus, Glienicker Strasse 100, D-14109 Berlin, Germany, ou_persistent22              
3Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              

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Free keywords: ATP synthase; Bacteriorhodopsin; Respiratory chain; Oxidative phosphorylation; β-amyloid peptide
 Abstract: By the elucidation of high-resolution structures the view of the bioenergetic processes has become more precise. But in the face of these fundamental advances, many problems are still unresolved. We have examined a variety of aspects of energy-transducing membranes from large protein complexes down to the level of protons and functional relevant picosecond protein dynamics. Based on the central role of the ATP synthase for supplying the biological fuel ATP, one main emphasis was put on this protein complex from both chloroplast and mitochondria. In particular the stoichiometry of protons required for the synthesis of one ATP molecule and the supramolecular organisation of ATP synthases were examined. Since formation of supercomplexes also concerns other complexes of the respiratory chain, our work was directed to unravel this kind of organisation, e.g. of the OXPHOS supercomplex I1III2IV1, in terms of structure and function. Not only the large protein complexes or supercomplexes work as key players for biological energy conversion, but also small components as quinones which facilitate the transfer of electrons and protons. Therefore, their location in the membrane profile was determined by neutron diffraction. Physico-chemical features of the path of protons from the generators of the electrochemical gradient to the ATP synthase, as well as of their interaction with the membrane surface, could be elucidated by time-resolved absorption spectroscopy in combination with optical pH indicators. Diseases such as Alzheimer's dementia (AD) are triggered by perturbation of membranes and bioenergetics as demonstrated by our neutron scattering studies.

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Language(s): eng - English
 Dates: 2009-02-202008-12-162009-02-202009-03-102009-06-01
 Publication Status: Published in print
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.bbabio.2009.02.028
PMID: 19281792
 Degree: -

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Title: Biochimica et Biophysica Acta, Bioenergetics
  Abbreviation : Biochim. Biophys. Acta, Bioenerg.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 1787 (6) Sequence Number: - Start / End Page: 657 - 671 Identifier: ISSN: 0005-2728
CoNE: https://pure.mpg.de/cone/journals/resource/954926938702_6