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  Lipids and membrane protein structures

Hunte, C., & Richers, S. (2008). Lipids and membrane protein structures. Current Opinion in Structural Biology, 18(4), 406-411. doi:10.1016/j.sbi.2008.03.008.

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 Creators:
Hunte, Carola1, Author
Richers, Sebastian2, Author           
Affiliations:
1Institute of Membrane and Systems Biology, University of Leeds, Leeds LS2 9JT, UK, ou_persistent22              
2Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              

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 Abstract: Membrane proteins do not work alone. The interaction of proteins with membrane lipids can be highly specific and is often important for full functional and structural integrity of the protein. Providing the appropriate lipid environment is of great importance for the purification and crystallisation of membrane proteins. The lipid content can be modulated by adjusting purification protocols or by adding back native or non-native lipids. Lipids can facilitate crystallisation by stabilising the protein and by providing lattice contacts. Of special interest is the crystallisation in lipidic cubic phase and with bicelles, as they appear to provide a membrane-like environment. These strategies have been instrumental for recent successful structure determinations of a human G-protein-coupled receptor, the β2-adrenergic receptor. Lipid supplementation can also help to obtain membrane protein structures in a native conformation, as shown for voltage-gated potassium channels. Membrane protein structures, especially those derived from lipid-enriched preparations, contain bound lipid molecules. Specific protein–lipid interactions not only require careful evaluation and interpretation, but also permit a directed approach to elucidate the structural and/or functional role of these interactions.

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Language(s): eng - English
 Dates: 2008-05-192008-08-01
 Publication Status: Published in print
 Pages: 6
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/j.sbi.2008.03.008
PMID: 18495472
 Degree: -

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Title: Current Opinion in Structural Biology
  Other : Curr. Opin. Struct. Biol.
Source Genre: Journal
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Publ. Info: Amsterdam : Elsevier
Pages: - Volume / Issue: 18 (4) Sequence Number: - Start / End Page: 406 - 411 Identifier: ISSN: 0959-440X
CoNE: https://pure.mpg.de/cone/journals/resource/954925578067