English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Agonist- and competitive antagonist-induced movement of loop 5 on the α subunit of the neuronal α4β4 nicotinic acetylcholine receptor

Mourot, A., Bamberg, E., & Rettinger, J. (2008). Agonist- and competitive antagonist-induced movement of loop 5 on the α subunit of the neuronal α4β4 nicotinic acetylcholine receptor. Journal of Neurochemistry, 105(2), 413-424. doi:10.1111/j.1471-4159.2007.05151.x.

Item is

Files

show Files

Locators

show

Creators

show
hide
 Creators:
Mourot, Alexandre1, Author           
Bamberg, Ernst1, Author           
Rettinger, Jürgen1, Author           
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              

Content

show
hide
Free keywords: -
 Abstract: Neuronal nicotinic acetylcholine receptors (nAChRs) are ligand-gated ion channels that rapidly convert a chemical signal into an electrical signal. Although the structure of the nAChR is quite well described, the coupling between agonist binding and channel gating is still under debate. In this study, we probed local conformational transitions on the neuronal alpha4beta4 nAChR by specifically tethering a conformation-sensitive fluorescent dye on alphaG98C located on loop 5 (L5), and simultaneously monitoring fluorescence intensity and current after expression in Xenopus oocytes. The potency of acetylcholine (ACh) was significantly higher in the cysteine mutant and further increased upon tetramethylrhodamine-6-maleimide labeling, suggesting a role of L5 in binding or gating. Structural reorganizations of L5 were shown to occur upon activation, as revealed by the fluorescence intensity increase during ACh exposure. Fluorescence changes were also detected at ACh concentrations lower than needed for current activation, suggesting a movement of L5 for a closed, resting or desensitized state. The competitive antagonist dihydro-beta-erythroidine also induced a movement of L5 although at concentrations significantly higher than needed for current inhibition. Consequently L5, located inside the lumen of the pentamer, plays a role in both activation and inhibition of the nAChR.

Details

show
hide
Language(s): eng - English
 Dates: 2007-112008-04
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 413801
DOI: 10.1111/j.1471-4159.2007.05151.x
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: Journal of Neurochemistry
  Other : J. Neurochem.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: New York : Raven Press [etc.]
Pages: - Volume / Issue: 105 (2) Sequence Number: - Start / End Page: 413 - 424 Identifier: ISSN: 0022-3042
CoNE: https://pure.mpg.de/cone/journals/resource/954925416956_1