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  The oligomeric state of c rings from cyanobacterial F-ATP synthases varies from 13 to 15.

Pogoryelov, D., Reichen, C., Klyszejko, A. L., Brunisholz, R., Muller, D. J., Dimroth, P., et al. (2007). The oligomeric state of c rings from cyanobacterial F-ATP synthases varies from 13 to 15. Journal of Bacteriology, 189(16), 5895-5902. doi:10.1128/JB.00581-07.

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 Creators:
Pogoryelov, Denys1, Author           
Reichen, Christian2, Author
Klyszejko, Adriana L.1, Author           
Brunisholz, René3, Author
Muller, Daniel J.4, Author
Dimroth, Peter1, Author
Meier, Thomas1, Author           
Affiliations:
1Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              
2Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zurich, CH-8093 Zurich, Switzerland, ou_persistent22              
3René Brunisholz Functional Genomics Center Zurich, University of Zurich and ETH, CH-8057 Zurich, Switzerland, ou_persistent22              
4Center for Biotechnology, University of Technology, 01307 Dresden, Germany , ou_persistent22              

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 Abstract: We isolated the c rings of F-ATP synthases from eight cyanobacterial strains belonging to four different taxonomic classes (Chroococcales, Nostocales, Oscillatoriales, and Gloeobacteria). These c rings showed different mobilities on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE), probably reflecting their molecular masses. This supposition was validated with the previously characterized c11, c14, and c15 rings, which migrated on SDS-PAGE in proportion to their molecular masses. Hence, the masses of the cyanobacterial c rings can conveniently be deduced from their electrophoretic mobilities and, together with the masses of the c monomers, allow the calculation of the c ring stoichiometries. The method is a simple and fast way to determine stoichiometries of SDS-stable c rings and hence a convenient means to unambiguously determine the ion-to-ATP ratio, a parameter reflecting the bioenergetic efficacy of F-ATP synthases. AFM imaging was used to prove the accuracy of the method and confirmed that the c ring of Synechococcus elongatus SAG 89.79 is a tridecameric oligomer. Despite the high conservation of the c-subunit sequences from cyanobacterial strains from various environmental groups, the stoichiometries of their c rings varied between c13 and c15. This systematic study of the c-ring stoichiometries suggests that variability of c-ring sizes might represent an adaptation of the individual cyanobacterial species to their particular environmental and physiological conditions. Furthermore, the two new examples of c15 rings underline once more that an F1/Fo symmetry mismatch is not an obligatory feature of all F-ATP synthases.

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Language(s): eng - English
 Dates: 2007-04-162007-05-222007-06-012007-08-15
 Publication Status: Published in print
 Pages: 8
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1128/JB.00581-07
PMID: 17545285
PMC: PMC1952053
 Degree: -

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Title: Journal of Bacteriology
  Other : J. Bacteriol.
Source Genre: Journal
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Publ. Info: Washington, DC : American Society for Microbiology (ASM)
Pages: - Volume / Issue: 189 (16) Sequence Number: - Start / End Page: 5895 - 5902 Identifier: ISSN: 0021-9193
CoNE: https://pure.mpg.de/cone/journals/resource/954925410823