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  A tridecameric c ring of the adenosine triphosphate (ATP) synthase from the thermoalkaliphilic Bacillus sp. strain TA2.A1 facilitates ATP synthesis at low electrochemical proton potential

Meier, T., Morgner, N., Matthies, D., Pogoryelov, D., Keis, S., Cook, G. M., et al. (2007). A tridecameric c ring of the adenosine triphosphate (ATP) synthase from the thermoalkaliphilic Bacillus sp. strain TA2.A1 facilitates ATP synthesis at low electrochemical proton potential. Molecular Microbiology, 65(5), 1181-1192. doi:10.1111/j.1365-2958.2007.05857.x.

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 Creators:
Meier, Thomas1, Author           
Morgner, Nina2, Author
Matthies, Doreen1, Author           
Pogoryelov, Denys1, Author           
Keis, Stefanie3, Author
Cook, Gregory M.3, Author
Dimroth, Peter4, Author
Brutschy, Bernhard2, Author
Affiliations:
1Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              
2Institute of Physical and Theoretical Chemistry, Johann Wolfgang Goethe-Universität, 60438 Frankfurt am Main, Germany, ou_persistent22              
3Department of Microbiology and Immunology, Otago School of Medical Sciences, University of Otago, Dunedin, New Zealand, ou_persistent22              
4Institute of Microbiology, Eidgenössische Technische Hochschule (ETH) Zürich, CH-8093 Zürich, Switzerland, ou_persistent22              

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 Abstract: Despite the thermodynamic problem imposed on alkaliphilic bacteria of synthesizing adenosine triphosphate (ATP) against a large inverted pH gradient and consequently a low electrochemical proton potential, these bacteria still utilize a proton-coupled F1Fo-ATP synthase to synthesize ATP. One potential solution to this apparent thermodynamic problem would be the operation of a larger oligomeric c ring, which would raise the ion to ATP ratio, thus facilitating the conversion of a low electrochemical potential into a significant phosphorylation potential. To address this hypothesis, we have purified the oligomeric c ring from the thermoalkaliphilic bacterium Bacillus sp. strain TA2.A1 and determined the number of c-subunits using a novel mass spectrometry method, termed ‘laser-induced liquid bead ion desorption’ (LILBID). This technique allows the mass determination of non-covalently assembled, detergent-solubilized membrane protein complexes, and hence enables an accurate determination of c ring stoichiometries. We show that the Bacillus sp. strain TA2.A1 ATP synthase harbours a tridecameric c ring. The operation of a c ring with 13 subunits renders the thermodynamic problem of ATP synthesis at alkaline pH less severe and may represent a strategy for ATP synthesis at low electrochemical potential.

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Language(s): eng - English
 Dates: 2007-06-262007-07-272007-09-01
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1111/j.1365-2958.2007.05857.x
PMID: 17645441
 Degree: -

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Title: Molecular Microbiology
  Other : Mol. Microbiol.
Source Genre: Journal
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Publ. Info: Oxford : Blackwell Science
Pages: - Volume / Issue: 65 (5) Sequence Number: - Start / End Page: 1181 - 1192 Identifier: ISSN: 0950-382X
CoNE: https://pure.mpg.de/cone/journals/resource/954925574950