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  NMDAR channel segments forming the extracellular vestibule inferred from the accessibility of substituted cysteines

Beck, C., Wollmuth, L. P., Seeburg, P. H., Sakmann, B., & Kuner, T. (1999). NMDAR channel segments forming the extracellular vestibule inferred from the accessibility of substituted cysteines. Neuron, 22(3), 559-570. doi:10.1016/S0896-6273(00)80710-2.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-6048-5 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002D-A96B-8
Genre: Journal Article
Alternative Title : NMDAR channel segments forming the extracellular vestibule inferred from the accessibility of substituted cysteines

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Neuron_22_1999_559.pdf (Any fulltext), 240KB
 
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 Creators:
Beck, Christine1, Author              
Wollmuth, Lonnie P.2, Author              
Seeburg, Peter H.1, Author              
Sakmann, Bert2, Author              
Kuner, Thomas1, 2, Author              
Affiliations:
1Department of Molecular Neurobiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497704              
2Department of Cell Physiology, Max Planck Institute for Medical Research, Max Planck Society, ou_1497701              

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 Abstract: In NMDA receptor channels, the M2 loop forms the narrow constriction and the cytoplasmic vestibule. The identity of an extracellular vestibule leading toward the constriction remained unresolved. Using the substituted cysteine accessibility method (SCAM), we identified channel-lining residues of the NR1 subunit in the region preceding M1 (preM1), the C-terminal part of M3 (M3C), and the N-terminal part of M4 (M4N). These residues are located on the extracellular side of the constriction and, with one exception, are exposed to the pore independently of channel activation, suggesting that the gate is at the constriction or further cytoplasmic to it. Permeation of Ca2+ ions was decreased by mutations in M3C and M4N, but not by mutations in preM1, suggesting a functionally distinct contribution of the segments to the extracellular vestibule of the NMDA receptor channel

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Language(s): eng - English
 Dates: 1999-01-201998-12-011999-01-201999-03-01
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Neuron
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 22 (3) Sequence Number: - Start / End Page: 559 - 570 Identifier: ISSN: 0896-6273
CoNE: https://pure.mpg.de/cone/journals/resource/954925560565