Expression, purification, crystallization and preliminary X-ray analysis of 
strictosidine glucosidase, an enzyme initiating biosynthetic pathways to a 
unique diversity of indole alkaloid skeletons

Barleben, Leif; Ma, Xueyan; Koepke, Jürgen; Peng, Guohong; Michel, Hartmut; Stöckigt, Joachim

doi:10.1016/j.bbapap.2004.09.026

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Expression, purification, crystallization and preliminary X-ray analysis of strictosidine glucosidase, an enzyme initiating biosynthetic pathways to a unique diversity of indole alkaloid skeletons

Barleben, L., Ma, X., Koepke, J., Peng, G., Michel, H., & Stöckigt, J. (2005). Expression, purification, crystallization and preliminary X-ray analysis of strictosidine glucosidase, an enzyme initiating biosynthetic pathways to a unique diversity of indole alkaloid skeletons. Proteins & Proteomics, 89-92. doi:10.1016/j.bbapap.2004.09.026.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-DA3E-1 Version Permalink: https://hdl.handle.net/21.11116/0000-000B-A286-6

Genre: Journal Article

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Creators:
Barleben, Leif¹, Author
Ma, Xueyan¹, Author
Koepke, Jürgen², Author
Peng, Guohong², Author
Michel, Hartmut², Author
Stöckigt, Joachim¹, Author

Affiliations:
1Department of Pharmaceutical Biology, Institute of Pharmacy, Johannes Gutenberg-University Mainz, Staudinger Weg 5, D-55099 Mainz, Germany, ou_persistent22
2Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290

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Free keywords: Strictosidine β-d-glucosidase; Cloning and purification; Crystallization; X-ray analysis; Rauvolfia serpentina

Abstract: Strictosidine beta-D-glucosidase, a plant enzyme initiating biosynthetic pathways to about 2000 monoterpenoid indole alkaloids with an extremely large number of various carbon skeletons, has been functionally expressed in Escherichia coli and purified to homogeneity in mg scale. Crystals suitable for X-ray analysis were found by robot-mediated screening. Using the hanging-drop technique, optimum conditions were 0.3 M ammonium sulfate, 0.1 M sodium acetate, pH 4.6 and PEG 4000 (10%) as precipitant buffer. The crystals of strictosidine glucosidase belong to the space group P42₁2 with unit cell dimensions of a=157.63, c=103.59 A and diffract X-rays to 2.48-A resolution

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Language(s): eng - English

Dates: Modified: 2004-09-20Submitted: 2004-07-20Accepted: 2004-09-24Published Online: 2004-10-13Date issued: 2005-02-14

Publication Status: Issued

Pages: 4

Publishing info: -

Table of Contents: -

Rev. Type: Peer

Identifiers: DOI: 10.1016/j.bbapap.2004.09.026
PMID: 15680242

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Title: Proteins & Proteomics

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Pages: - Volume / Issue: - Sequence Number: - Start / End Page: 89 - 92 Identifier: -

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Title: Biochimica et Biophysica Acta-Proteins and Proteomics

Other : BBA-Proteins Proteomics

Source Genre: Journal

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Publ. Info: Amsterdam : Elsevier

Pages: - Volume / Issue: 1747 (1) Sequence Number: - Start / End Page: 89 - 92 Identifier: ISSN: 1570-9639
CoNE: https://pure.mpg.de/cone/journals/resource/954926938702_5