Vinorine synthase from Rauvolfia: the first example of crystallization and 
preliminary X-ray diffraction analysis of an enzyme of the BAHD superfamily

Ma, Xueyan; Koepke, Jürgen; Bayer, Anja; Linhard, Verena; Fritzsch, Günter; Zhang, Bin; Michel, Hartmut; Stöckigt, Joachim

doi:10.1016/j.bbapap.2004.06.011

Local TagsRelease HistoryDetailsSummary

Vinorine synthase from Rauvolfia: the first example of crystallization and preliminary X-ray diffraction analysis of an enzyme of the BAHD superfamily

Ma, X., Koepke, J., Bayer, A., Linhard, V., Fritzsch, G., Zhang, B., et al. (2004). Vinorine synthase from Rauvolfia: the first example of crystallization and preliminary X-ray diffraction analysis of an enzyme of the BAHD superfamily. Biochimica et Biophysica Acta-Proteins and Proteomics, 1701(1-2), 129-132. doi:10.1016/j.bbapap.2004.06.011.

Item is Released

show all hide all

Basic

show hide

Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-DAC7-D Version Permalink: https://hdl.handle.net/21.11116/0000-000B-A2EC-4

Genre: Journal Article

Files

show Files

Locators

show

Creators

show

hide

Creators:
Ma, Xueyan¹, Author
Koepke, Jürgen², Author
Bayer, Anja¹, Author
Linhard, Verena², Author
Fritzsch, Günter², Author
Zhang, Bin³, Author
Michel, Hartmut², Author
Stöckigt, Joachim¹, Author

Affiliations:
1Department of Pharmaceutical Biology, Institute of Pharmacy, Johannes Gutenberg-University Mainz, Staudinger Weg 5, 55099 Mainz, Germany, ou_persistent22
2Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290
3Institute of Physical Chemistry, Johannes Gutenberg-University Mainz, Welderweg 11-15, 55099 Mainz, Germany, ou_persistent22

Content

show

hide

Free keywords: Acetyltransferase;Vinorine synthase; BAHD superfamily; Crystallization; Ajmaline biosynthesis

Abstract: Crystals of vinorine synthase (VS) from medicinal plant Rauvolfia serpentina expressed in Escherichia coli have been obtained by the hanging-drop technique at 305 K with ammonium sulfate and PEG 400 as precipitants. The enzyme is involved in the biosynthesis of the antiarrhythmic drug ajmaline and is a member of the BAHD superfamily of acyltransferases. So far, no three-dimensional structure of a member of this enzyme family is known. The crystals belong to the space group P2₁2₁2₁ with cell dimensions of a=82.3 A, b=89.6 A and c=136.2 A. Under cryoconditions (120 K), a complete data set up to 2.8 A was collected at a synchrotron source.

Details

show

hide

Language(s): eng - English

Dates: Modified: 2004-06-22Submitted: 2004-05-05Published Online: 2004-07-25Date issued: 2004-09-01

Publication Status: Issued

Pages: 4

Publishing info: -

Table of Contents: -

Rev. Type: Peer

Identifiers: DOI: 10.1016/j.bbapap.2004.06.011
PMID: 15450182

Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show

hide

Title: Biochimica et Biophysica Acta-Proteins and Proteomics

Other : BBA-Proteins Proteomics

Source Genre: Journal

Creator(s):

Affiliations:

Publ. Info: Amsterdam : Elsevier

Pages: - Volume / Issue: 1701 (1-2) Sequence Number: - Start / End Page: 129 - 132 Identifier: ISSN: 1570-9639
CoNE: https://pure.mpg.de/cone/journals/resource/954926938702_5