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  Charge displacements during ATP-hydrolysis and synthesis of the Na+ -transporting FoF1-ATPase of Ilyobacter tartaricus

Burzik, C., Kaim, G., Dimroth, P., Bamberg, E., & Fendler, K. (2003). Charge displacements during ATP-hydrolysis and synthesis of the Na+ -transporting FoF1-ATPase of Ilyobacter tartaricus. Biophysical Journal, 85(3), 2044-2054. doi:10.1016/S0006-3495(03)74632-3.

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 Creators:
Burzik, Christiane1, Author           
Kaim, Georg2, Author
Dimroth, Peter2, Author
Bamberg, Ernst1, 3, Author           
Fendler, Klaus1, Author           
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              
2Eidgenössische Technische Hochschule Zürich, CH-8092 Zürich, Switzerland, ou_persistent22              
3Goethe-Universität Frankfurt, 60439 Frankfurt am Main, Germany, ou_persistent22              

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 Abstract: Transient electrical currents generated by the Na+-transporting FoF1-ATPase of Ilyobacter tartaricus were observed in the hydrolytic and synthetic mode of the enzyme. Two techniques were applied: a photochemical ATP concentration jump on a planar lipid membrane and a rapid solution exchange on a solid supported membrane. We have identified an electrogenic reaction in the reaction cycle of the FoF1-ATPase that is related to the translocation of the cation through the membrane bound Fo subcomplex of the ATPase. In addition, we have determined rate constants for the process: For ATP hydrolysis this reaction has a rate constant of 15–30 s−1 if H+ is transported and 30–60 s−1 if Na+ is transported. For ATP synthesis the rate constant is 50–70 s−1.

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Language(s): eng - English
 Dates: 2002-08-162003-04-032009-01-062003-09-01
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1016/S0006-3495(03)74632-3
 Degree: -

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Title: Biophysical Journal
  Other : Biophys. J.
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 85 (3) Sequence Number: - Start / End Page: 2044 - 2054 Identifier: ISSN: 0006-3495
CoNE: https://pure.mpg.de/cone/journals/resource/954925385117