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  Associations between light-harvesting complexes and Photosystem II from Marchantia polymorpha L. determined by two- and three-dimensional electron microscopy

Harrer, R. (2003). Associations between light-harvesting complexes and Photosystem II from Marchantia polymorpha L. determined by two- and three-dimensional electron microscopy. Photosynthesis Research, 75(3), 249-258. doi:10.1023/A:1023952832255.

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 Creators:
Harrer, Roswitha1, Author              
Affiliations:
1Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              

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Free keywords: electron microscopy; light-harvesting complexes; liverworts; Marchantia polymorpha; Photosystem II; random conical tilt; single-particle analysis; three-dimensional reconstruction
 Abstract: Assemblies of Photosystem II and light-harvesting proteins were purified from the liverwort Marchantia polymorpha and investigated by two- and three-dimensional transmission electron microscopy of negatively stained specimens. By single-particle analysis, it was determined that about 25% of the particles are rectangular or slightly S-shaped with dimensions of 285 Å in length, 144 Å in width, 84 Å in height, while the membrane part is about 52 Å thick. This structure reveals the same architecture as that of a Photosystem II-light-harvesting assembly from seed plants. An overlay of the projection structure of the liverwort's complex with a projection structure deduced from stained trimeric LHC II crystals from pea confirmed the locations of trimeric LHC II within the liverwort's complex. Remarkably tight associations of LHC II and other chlorophyll a/b binding proteins with the PS II core complex are observed. More than 50% of the Photosystem II particles from the liverwort carry one or two additional masses. These extra masses are found to consist of an additional LHC II trimer and probably a chlorophyll a/b binding protein. For the first time, a three-dimensional structure of such a large assembly is defined.

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Language(s): eng - English
 Dates: 2002-12-302002-10-102003-03-01
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1023/A:1023952832255
 Degree: -

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Title: Photosynthesis Research
Source Genre: Journal
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Publ. Info: Hague : W. Junk
Pages: - Volume / Issue: 75 (3) Sequence Number: - Start / End Page: 249 - 258 Identifier: ISSN: 0166-8595
CoNE: https://pure.mpg.de/cone/journals/resource/954925482637