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  Reduction of cytochrome c oxidase by a second electron leads to proton translocation

Ruitenberg, M., Kannt, A., Bamberg, E., Fendler, K., & Michel, H. (2002). Reduction of cytochrome c oxidase by a second electron leads to proton translocation. Nature, 417(6884), 99-102. doi:10.1038/417099a.

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 Creators:
Ruitenberg, Maarten1, Author              
Kannt, Aimo2, Author              
Bamberg, Ernst1, Author              
Fendler, Klaus1, Author              
Michel, Hartmut2, Author              
Affiliations:
1Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              
2Department of Molecular Membrane Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068290              

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Free keywords: Bo ubiquinol oxidase; Paracoccus-denitrificans; Escherichia-coli; Subunit-i; Site ; State; Oxidase; Respiration; Mitochondria; Bacteria
 Abstract: Cytochrome c oxidase, the terminal enzyme of cellular respiration in mitochondria and many bacteria, reduces O-2 to water. This four-electron reduction process is coupled to translocation (pumping) of four protons across the mitochondrial or bacterial membrane(1); however, proton pumping is poorly understood. Proton pumping was thought to be linked exclusively to the oxidative phase, that is, to the transfer of the third and fourth electron(2). Upon re-evaluation of these data, however, this proposal has been questioned(3,4), and a transport mechanism including proton pumping in the reductive phase-that is, during the transfer of the first two electrons-was suggested. Subsequently, additional studies reported that proton pumping during the reductive phase can occur, but only when it is immediately preceded by an oxidative phase(5). To help clarify the issue we have measured the generation of the electric potential across the membrane, starting from a defined one-electron reduced state. Here we show that a second electron transfer into the enzyme leads to charge translocation corresponding to pumping of one proton without necessity for a preceding turnover. [References: 22]

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Language(s): eng - English
 Dates: 2001-07-302002-02-182002-05-02
 Publication Status: Published in print
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/417099a
 Degree: -

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Title: Nature
  Abbreviation : Nature
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 417 (6884) Sequence Number: - Start / End Page: 99 - 102 Identifier: ISSN: 0028-0836
CoNE: https://pure.mpg.de/cone/journals/resource/954925427238