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  Protein translocase of the outer mitochondrial membrane: role of import receptors in the structural organization of the TOM complex

Model, K., Prinz, T., Ruiz, T., Radermacher, M., Krimmer, T., Kühlbrandt, W., et al. (2002). Protein translocase of the outer mitochondrial membrane: role of import receptors in the structural organization of the TOM complex. Journal of Molecular Biology, 316(3), 657-666. doi:10.1006/jmbi.2001.5365.

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 Creators:
Model, Kirstin1, Author           
Prinz, T.2, Author
Ruiz, Teresa1, Author           
Radermacher, M.1, Author           
Krimmer, T.2, Author
Kühlbrandt, Werner1, Author           
Pfanner, N.2, Author
Meisinger, C.2, Author
Affiliations:
1Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              
2Institut für Biochemie und Molekularbiologie, Universität Freiburg, Freiburg, Germany, ou_persistent22              

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Free keywords: Fungal Proteins/me [Metabolism]; Gene Deletion; *Intracellular Membranes/en [Enzymology]; Membrane Proteins/ch [Chemistry]; Membrane Proteins/ge [Genetics]; *Membrane Proteins/me [Metabolism]; Membrane Proteins/ul [Ultrastructure]; Membrane Transport Proteins/ch [Chemistry]; Membrane Transport Proteins/ge [Genetics]; *Membrane Transport Proteins/me [Metabolism]; Membrane Transport Proteins/ul [Ultrastructure]; Microscopy, Electron; *Mitochondria/en [Enzymology]; Mitochondria/ge [Genetics]; Mitochondrial Proteins/ch [Chemistry]; Mitochondrial Proteins/ge [Genetics]; *Mitochondrial Proteins/me [Metabolism]; Mitochondrial Proteins/ul [Ultrastructure]; Neurospora/en [Enzymology]; Protein Precursors/me [Metabolism]; Protein Structure, Quaternary; Protein Transport; *Saccharomyces cerevisiae/cy [Cytology]; *Saccharomyces cerevisiae/en [Enzymology]; Saccharomyces cerevisiae/ge [Genetics]; Saccharomyces cerevisiae Proteins/ch [Chemistry]; Saccharomyces cerevisiae Proteins/ge [Genetics]; *Saccharomyces cerevisiae Proteins/me [Metabolism]; Saccharomyces cerevisiae Proteins/ul [Ultrastructure]; Support, Non-U.S. Gov't; Support, U.S. Gov't, Non-P.H.S.
 Abstract: The mitochondrial outer membrane contains a multi-subunit machinery responsible for the specific recognition and translocation of precursor proteins. This translocase of the outer membrane (TOM) consists of three receptor proteins, Tom20, Tom22 and Tom70, the channel protein Tom40, and several small Tom proteins. Single-particle electron microscopy analysis of the Neurospora TOM complex has led to different views with two or three stain-filled centers resembling channels. Based on biochemical and electron microscopy studies of the TOM complex isolated from yeast mitochondria, we have discovered the molecular reason for the different number of channel-like structures. The TOM complex from wild-type yeast contains up to three stain-filled centers, while from a mutant yeast selectively lacking Tom20, the TOM complex particles contain only two channel-like structures. From mutant mitochondria lacking Tom22, native electrophoresis separates an approximately 80 kDa subcomplex that consists of Tom40 only and is functional for accumulation of a precursor protein. We conclude that while Tom40 forms the import channels, the two receptors Tom22 and Tom20 are required for the organization of Tom40 dimers into larger TOM structures. Copyright 2002 Elsevier Science Ltd.

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Language(s): eng - English
 Dates: 2002
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: eDoc: 12094
DOI: 10.1006/jmbi.2001.5365
 Degree: -

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Title: Journal of Molecular Biology
  Other : JMB
  Abbreviation : J. Mol. Biol.
Source Genre: Journal
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Publ. Info: Elsevier
Pages: - Volume / Issue: 316 (3) Sequence Number: - Start / End Page: 657 - 666 Identifier: ISSN: 0022-2836
CoNE: https://pure.mpg.de/cone/journals/resource/0022-2836