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  Preparation of active enzyme samples for IR studies of Na+, K+-ATPase

Thoenges, D., Tscherp, C., Grell, E., & Barth, A. (2002). Preparation of active enzyme samples for IR studies of Na+, K+-ATPase. Biopolymers, 67(4-5), 271-274. doi:10.1002/bip.10108.

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Item Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-DCA7-5 Version Permalink: https://hdl.handle.net/21.11116/0000-0009-2C4F-F
Genre: Journal Article

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 Creators:
Thoenges, Detlef1, Author
Tscherp, Christian1, Author
Grell, Ernst2, Author           
Barth, Andreas1, Author
Affiliations:
1Institut für Biophysik, Johann Wolfgang Goethe-Universität, Theodor-Stern-Kai 7/Haus 74, 60590 Frankfurt am Main, Germany, ou_persistent22              
2Department of Biophysical Chemistry, Max Planck Institute of Biophysics, Max Planck Society, ou_2068289              

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Free keywords: Na+/K+-ATPase; IR spectroscopy; Fourier transform IR; sample prepa- ration; caged ATP
 Abstract: In the case of the integral membrane protein Na+/K+-ATPase, preparation of highly concentrated samples for IR difference spectroscopy often leads to inactivation of the enzyme. Therefore, we compared the activity of Na+/K+-ATPase using different techniques of sample preparation. The loss of activity can be minimized by cooling the sample to 10°C and by the addition of glycerol and dithiothreitol. The activity of Na+/K+-ATPase isolated from pig kidney is independent of the protein concentration whereas the enzyme from shark rectal gland is inactivated at concentrations above 1 μg/μL and is thus unsuitable for IR experiments.

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Language(s): eng - English
 Dates: 2001-10-172001-09-082001-11-072002-04-072002-05-09
 Publication Status: Issued
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/bip.10108
PMID: 12012445
 Degree: -

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Title: Biopolymers
  Other : Biopolym.
Source Genre: Journal
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Publ. Info: New York, NY : John Wiley & Sons, Inc.
Pages: - Volume / Issue: 67 (4-5) Sequence Number: - Start / End Page: 271 - 274 Identifier: ISSN: 0006-3525
CoNE: https://pure.mpg.de/cone/journals/resource/110992357253752