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  RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus.

Staals, R. H., Zhu, Y., Taylor, D. W., Kornfeld, J. E., Sharma, K., Barendregt, A., et al. (2014). RNA targeting by the type III-A CRISPR-Cas Csm complex of Thermus thermophilus. Molecular Cell, 56(4), 518-530. doi:10.1016/j.molcel.2014.10.005.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-63C9-B Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0029-040D-1
Genre: Journal Article

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 Creators:
Staals, R. H., Author
Zhu, Y., Author
Taylor, D. W., Author
Kornfeld, J. E., Author
Sharma, K.1, Author              
Barendregt, A., Author
Koehorst, J. F., Author
Vlot, M., Author
Neupane, N., Author
Varossieau, K., Author
Sakamoto, K., Author
Suzuki, T., Author
Dohmae, N., Author
Yokoyama, S., Author
Schaap, P. J., Author
Urlaub, H.1, Author              
Heck, A. J. R., Author
Nogales, E., Author
Doudna, J. A., Author
Shinkai, A., Author
van der Oost, J., Author more..
Affiliations:
1Research Group of Bioanalytical Mass Spectrometry, MPI for Biophysical Chemistry, Max Planck Society, ou_578613              

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 Abstract: CRISPR-Cas is a prokaryotic adaptive immune system that provides sequence-specific defense against foreign nucleic acids. Here we report the structure and function of the effector complex of the Type III-A CRISPR-Cas system of Thermus thermophilus: the Csm complex (TtCsm). TtCsm is composed of five different protein subunits (Csm1–Csm5) with an uneven stoichiometry and a single crRNA of variable size (35–53 nt). The TtCsm crRNA content is similar to the Type III-B Cmr complex, indicating that crRNAs are shared among different subtypes. A negative stain EM structure of the TtCsm complex exhibits the characteristic architecture of Type I and Type III CRISPR-associated ribonucleoprotein complexes. crRNA-protein crosslinking studies show extensive contacts between the Csm3 backbone and the bound crRNA. We show that, like TtCmr, TtCsm cleaves complementary target RNAs at multiple sites. Unlike Type I complexes, interference by TtCsm does not proceed via initial base pairing by a seed sequence.

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Language(s): eng - English
 Dates: 2014-11-062014-11-20
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.molcel.2014.10.005
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Title: Molecular Cell
Source Genre: Journal
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Pages: - Volume / Issue: 56 (4) Sequence Number: - Start / End Page: 518 - 530 Identifier: -