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  Annotation of genomics data using bidirectional hidden Markov models unveils variations in Pol II transcription cycle.

Zacher, B., Lidschreiber, M., Cramer, P., Gagneur, J., & Tresch, A. (2014). Annotation of genomics data using bidirectional hidden Markov models unveils variations in Pol II transcription cycle. Molecular Systems Biology, 10(12): 768. doi:10.15252/msb.20145654.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-646A-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CD0D-F
Genre: Journal Article

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 Creators:
Zacher, B., Author
Lidschreiber, M.1, Author              
Cramer, P.1, Author              
Gagneur, J., Author
Tresch, A., Author
Affiliations:
1Department of Molecular Biology, MPI for Biophysical Chemistry, Max Planck Society, ou_1863498              

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Free keywords: bidirectional hidden Markov model; chromatin marks; genome annotation; RNA transcription cycle
 Abstract: DNA replication, transcription and repair involve the recruitment of protein complexes that change their composition as they progress along the genome in a directed or strand‐specific manner. Chromatin immunoprecipitation in conjunction with hidden Markov models (HMMs) has been instrumental in understanding these processes, as they segment the genome into discrete states that can be related to DNA‐associated protein complexes. However, current HMM‐based approaches are not able to assign forward or reverse direction to states or properly integrate strand‐specific (e.g., RNA expression) with non‐strand‐specific (e.g., ChIP) data, which is indispensable to accurately characterize directed processes. To overcome these limitations, we introduce bidirectional HMMs which infer directed genomic states from occupancy profiles de novo. Application to RNA polymerase II‐associated factors in yeast and chromatin modifications in human T cells recovers the majority of transcribed loci, reveals gene‐specific variations in the yeast transcription cycle and indicates the existence of directed chromatin state patterns at transcribed, but not at repressed, regions in the human genome. In yeast, we identify 32 new transcribed loci, a regulated initiation–elongation transition, the absence of elongation factors Ctk1 and Paf1 from a class of genes, a distinct transcription mechanism for highly expressed genes and novel DNA sequence motifs associated with transcription termination. We anticipate bidirectional HMMs to significantly improve the analyses of genome‐associated directed processes.

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Language(s): eng - English
 Dates: 2014-12-19
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: 10.15252/msb.20145654
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Title: Molecular Systems Biology
Source Genre: Journal
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Pages: 17 Volume / Issue: 10 (12) Sequence Number: 768 Start / End Page: - Identifier: -