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  2000-fold parallelized dual-color STED fluorescence nanoscopy.

Bergermann, F., Alber, L., Sahl, S. J., Engelhardt, S., & Hell, S. W. (2015). 2000-fold parallelized dual-color STED fluorescence nanoscopy. Optics Express, 23(1), 211-223. doi:10.1364/OE.23.000211.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-6D80-A Version Permalink: http://hdl.handle.net/21.11116/0000-0002-A719-6
Genre: Journal Article

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2082322.pdf (Publisher version), 4MB
 
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 Creators:
Bergermann, F., Author
Alber, L., Author
Sahl, S. J.1, Author              
Engelhardt, S., Author
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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 Abstract: Stimulated Emission Depletion (STED) nanoscopy enables multi-color fluorescence imaging at the nanometer scale. Its typical single-point scanning implementation can lead to long acquisition times. In order to unleash the full spatiotemporal resolution potential of STED nanoscopy, parallelized scanning is mandatory. Here we present a dual-color STED nanoscope utilizing two orthogonally crossed standing light waves as a fluorescence switch-off pattern, and providing a resolving power down to 30 nm. We demonstrate the imaging capabilities in a biological context for immunostained vimentin fibers in a circular field of view of 20 µm diameter at 2000-fold parallelization (i.e. 2000 “intensity minima”). The technical feasibility of massively parallelizing STED without significant compromises in resolution heralds video-rate STED nanoscopy of large fields of view, pending the availability of suitable high-speed detectors.

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Language(s): eng - English
 Dates: 2015-01-052015-01-12
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1364/OE.23.000211
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Title: Optics Express
Source Genre: Journal
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Pages: - Volume / Issue: 23 (1) Sequence Number: - Start / End Page: 211 - 223 Identifier: -