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  Excluded-Volume Effects in Living Cells

Gnutt, D., Gao, M., Brylski, O., Heyden, M., & Ebbinghaus, S. (2015). Excluded-Volume Effects in Living Cells. Angewandte Chemie, International Edition, 54(8), 2548-2551. doi:10.1002/anie.201409847.

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anie_201409847_sm_miscellaneous_information.pdf (Supplementary material), 824KB
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2014
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Wiley-VCH 69451 Weinheim, Germany
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 Creators:
Gnutt, David1, Author
Gao, Mimi1, Author
Brylski, Oliver1, Author
Heyden, Matthias2, Author           
Ebbinghaus, Simon1, Author
Affiliations:
1Department of Physical Chemistry II, Ruhr University Bochum, Universitätsstrasse 150, 44801 Bochum (Germany), ou_persistent22              
2Research Group Heyden, Max-Planck-Institut für Kohlenforschung, Max Planck Society, ou_1950292              

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Free keywords: biophysics; biosensors; excluded-volume effect; FRET; macromolecular crowding
 Abstract: Biomolecules evolve and function in densely crowded and highly heterogeneous cellular environments. Such conditions are often mimicked in the test tube by the addition of artificial macromolecular crowding agents. Still, it is unclear if such cosolutes indeed reflect the physicochemical properties of the cellular environment as the in-cell crowding effect has not yet been quantified. We have developed a macromolecular crowding sensor based on a FRET-labeled polymer to probe the macromolecular crowding effect inside single living cells. Surprisingly, we find that excluded-volume effects, although observed in the presence of artificial crowding agents, do not lead to a compression of the sensor in the cell. The average conformation of the sensor is similar to that in aqueous buffer solution and cell lysate. However, the in-cell crowding effect is distributed heterogeneously and changes significantly upon cell stress. We present a tool to systematically study the in-cell crowding effect as a modulator of biomolecular reactions.

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Language(s): eng - English
 Dates: 2014-10-072014-12-292015-02-16
 Publication Status: Issued
 Pages: 4
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1002/anie.201409847
 Degree: -

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Title: Angewandte Chemie, International Edition
  Other : Angew. Chem., Int. Ed.
  Other : Angew. Chem. Int. Ed
  Other : Angewandte Chemie International Edition
  Abbreviation : Angew. Chem. Int. Ed
Source Genre: Journal
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Publ. Info: Weinheim : Wiley-VCH Verlag GmbH & Co. KGaA
Pages: - Volume / Issue: 54 (8) Sequence Number: - Start / End Page: 2548 - 2551 Identifier: CoNE: https://pure.mpg.de/cone/journals/resource/1433-7851