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  Environmental factors affecting the expression of type IV pilus genes as well as piliation of Thermus thermophilus

Salzer, R., Kern, T., Joos, F., & Averhoff, B. (2014). Environmental factors affecting the expression of type IV pilus genes as well as piliation of Thermus thermophilus. FEMS Microbiology Letters, 357(1), 56-62. doi:10.1111/1574-6968.12506.

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 Creators:
Salzer, Ralf1, Author
Kern, Timo1, Author
Joos, Friederike2, Author           
Averhoff, Beate1, Author
Affiliations:
1Molecular Microbiology & Bioenergetics, Institute of Molecular Biosciences, Goethe University Frankfurt, Frankfurt am Main, Germany, ou_persistent22              
2Department of Structural Biology, Max Planck Institute of Biophysics, Max Planck Society, ou_2068291              

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Free keywords: type IV pili; DNA translocator; gene regulation; natural competence; Thermus thermophilus
 Abstract: The thermophilic bacterium Thermus thermophilus HB27 is known for its highly efficient natural transformation system, which has become a model system to study the structure and function of DNA transporter in thermophilic bacteria. The DNA transporter is functionally linked to type IV pili (T4P), which are essential for twitching motility and adhesion to solid surfaces. However, the pilus structures themselves are dispensable for natural transformation. Here, we report that the cellular mRNA levels of the major structural subunit of the T4P, PilA4, are regulated by environmental factors. Growth of T. thermophilus in minimal medium or low temperature (55 °C) leads to a significant increase in pilA4 transcripts. In contrast, the transcript levels of the minor pilin pilA1 as well as other T4P genes are nearly unaffected. The elevated pilA4 mRNA levels are accompanied by an increase in piliation of the cells but not by elevated natural transformation frequencies. Hyperpiliation leads to increased adhesion to plastic surfaces. The increased cell-surface interactions are suggested to represent an adaptive response to temperature stress and may be advantageous for survival of T. thermophilus.

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Language(s): eng - English
 Dates: 2014-07-022014-08
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1111/1574-6968.12506
 Degree: -

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Title: FEMS Microbiology Letters
  Other : FEMS Microbiol. Lett.
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: Wiley
Pages: - Volume / Issue: 357 (1) Sequence Number: - Start / End Page: 56 - 62 Identifier: ISSN: 0378-1097
CoNE: https://pure.mpg.de/cone/journals/resource/954925484705