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  A potentially exhaustive strategy reveals two novel divergent myosins in Dictyostelium

Schwarz, E. C., Geissler, H., & Soldati, T. (1999). A potentially exhaustive strategy reveals two novel divergent myosins in Dictyostelium. Cell Biochemistry and Biophysics, 30(3), 413-435. doi:10.1007/BF02738122.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-8248-6 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-828C-E
Genre: Journal Article
Alternative Title : A potentially exhaustive strategy reveals two novel divergent myosins in Dictyostelium

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CellBiochemBiophys_30_1999_413.pdf (Any fulltext), 2MB
 
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 Creators:
Schwarz, Eva C.1, Author              
Geissler, Heidrun1, Author              
Soldati, Thierry2, Author              
Affiliations:
1Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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Free keywords: Cytoskeleton ; myosin ; PCR ; Dictyostelium discoideum
 Abstract: In recent years, the myosin superfamily has kept expanding at an explosive rate, but the understanding of their complex functions has been lagging. Therefore,Dictyostelium discoideum, a genetically and biochemically tractable eukaryotic amoeba, appears as a powerful model organism to investigate the involvement of the actomyosin cytoskeleton in a variety of cellular tasks. Because of the relatively high degree of functional redundancy, such studies would be greatly facilitated by the prior knowledge of the whole myosin repertoire in this organism. Here, we present a strategy based on PCR amplification using degenerate primers and followed by negative hybridization screening which led to the potentially exhaustive identification of members of the myosin family inD. discoideum. Two novel myosins were identified and their genetic loci mapped by hybridization to an ordered YAC library. Preliminary inspection ofmyoK andmyoM sequences revealed that, despite carrying most of the hallmarks of myosin motors, both molecules harbor features surprisingly divergent from most known myosins

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Language(s): eng - English
 Dates: 1999-10-01
 Publication Status: Published in print
 Pages: 23
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Degree: -

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Title: Cell Biochemistry and Biophysics
  Other : Cell Biochem Biophys
Source Genre: Journal
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Publ. Info: Totowa, NJ : Humana Press
Pages: - Volume / Issue: 30 (3) Sequence Number: - Start / End Page: 413 - 435 Identifier: ISSN: 1085-9195
CoNE: https://pure.mpg.de/cone/journals/resource/954927619054