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  Environmentally determined differences in the murine lung microbiota and their relation to alveolar architecture

Yun, Y., Srinivas, G., Künzel, S., Linnenbrink, M., Alnahas, S., Bruce, K. D., et al. (2014). Environmentally determined differences in the murine lung microbiota and their relation to alveolar architecture. PLoS One, 9(12): e113466. doi:DOI:10.1371/journal.pone.0113466.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-9106-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-9107-C
Genre: Journal Article

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 Creators:
Yun, Yeojun, Author
Srinivas, Girish1, Author              
Künzel, Sven2, Author              
Linnenbrink, Miriam2, Author              
Alnahas, Safa, Author
Bruce, Kenneth D., Author
Steinhoff, Ulrich, Author
Baines, John F.1, Author              
Schaible, Ulrich E., Author
Affiliations:
1Guest Group Evolutionary Genomics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445638              
2Department Evolutionary Genetics, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445635              

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 Abstract: Commensal bacteria control the micro-ecology of metazoan epithelial surfaces with pivotal effect on tissue homeostasis and host defense. In contrast to the upper respiratory tract, the lower respiratory tract of healthy individuals has largely been considered free of microorganisms. To understand airway micro-ecology we studied microbiota of sterilely excised lungs from mice of different origin including outbred wild mice caught in the natural environment or kept under non-specific-pathogen-free (SPF) conditions as well as inbred mice maintained in non-SPF, SPF or germ-free (GF) facilities. High-throughput pyrosequencing of reverse transcribed 16S rRNA revealed metabolically active murine lung microbiota in all but GF mice. The overall composition across samples was similar at the phylum and family level. However, species richness was significantly different between lung microbiota from SPF and non-SPF mice. Non-cultivatable Betaproteobacteria such as Ralstonia spp. made up the major constituents and were also confirmed by 16S rRNA gene cloning analysis. Additionally, Pasteurellaceae, Enterobacteria and Firmicutes were isolated from lungs of non-SPF mice. Bacterial communities were detectable by fluorescent in situ hybridization (FISH) at alveolar epithelia in the absence of inflammation. Notably, higher bacterial abundance in non-SPF mice correlated with more and smaller size alveolae, which was corroborated by transplanting Lactobacillus spp. lung isolates into GF mice. Our data indicate a common microbial composition of murine lungs, which is diversified through different environmental conditions and affects lung architecture. Identification of the microbiota of murine lungs will pave the path to study their influence on pulmonary immunity to infection and allergens using mouse models.

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Language(s): eng - English
 Dates: 2014-05-132014-10-272014-12-03
 Publication Status: Published online
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 Rev. Method: Peer
 Identifiers: DOI: DOI:10.1371/journal.pone.0113466
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Title: PLoS One
Source Genre: Journal
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Publ. Info: San Francisco, CA : Public Library of Science
Pages: 24 Volume / Issue: 9 (12) Sequence Number: e113466 Start / End Page: - Identifier: ISSN: 1932-6203
CoNE: /journals/resource/1000000000277850