Deutsch
 
Benutzerhandbuch Datenschutzhinweis Impressum Kontakt
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Protein crystal structure obtained at 2.9 Å resolution from injecting bacterial cells into an X-ray free-electron laser beam

Sawaya, M. R., Cascio, D., Gingery, M., Rodriguez, J., Goldschmidt, L., Colletier, J.-P., et al. (2014). Protein crystal structure obtained at 2.9 Å resolution from injecting bacterial cells into an X-ray free-electron laser beam. Proceedings of the National Academy of Sciences of the United States of America, 111(35), 12769-12774. doi:10.1073/pnas.1413456111.

Item is

Basisdaten

einblenden: ausblenden:
Datensatz-Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-951D-D Versions-Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-951E-B
Genre: Zeitschriftenartikel

Dateien

einblenden: Dateien
ausblenden: Dateien
:
PNAS_111_2014_12769.pdf (beliebiger Volltext), 852KB
 
Datei-Permalink:
-
Name:
PNAS_111_2014_12769.pdf
Beschreibung:
-
Sichtbarkeit:
Eingeschränkt (Max Planck Institute for Medical Research, Heidelberg; )
MIME-Typ / Prüfsumme:
application/pdf
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Externe Referenzen

einblenden:
ausblenden:
externe Referenz:
http://www.pnas.org/content/111/35/12769.full.pdf+html (beliebiger Volltext)
Beschreibung:
-
externe Referenz:
http://dx.doi.org/10.1073/pnas.1413456111 (beliebiger Volltext)
Beschreibung:
-

Urheber

einblenden:
ausblenden:
 Urheber:
Sawaya, Michael R., Autor
Cascio, Duilio, Autor
Gingery, Mari, Autor
Rodriguez, Jose, Autor
Goldschmidt, Lukasz, Autor
Colletier, Jacques-Philippe, Autor
Messerschmidt, Marc M., Autor
Boutet, Sébastien, Autor
Koglin, Jason E., Autor
Williams, Garth J., Autor
Brewster, Aaron S., Autor
Nass, Karol1, Autor              
Hattne, Johan, Autor
Botha, Sabine1, Autor              
Doak, Bruce1, Autor              
Shoeman, Robert L.1, Autor              
DePonte, Daniel P., Autor
Park, Hyun-Woo, Autor
Federici, Brian A., Autor
Sauter, Nicholas K., Autor
Schlichting, Ilme1, Autor              Eisenberg, David S., Autor mehr..
Affiliations:
1Department of Biomolecular Mechanisms, Max Planck Institute for Medical Research, Max Planck Society, ou_1497700              

Inhalt

einblenden:
ausblenden:
Schlagwörter: Cry3A insecticidal toxin; XFEL; serial femtosecond crystallography
 Zusammenfassung: It has long been known that toxins produced by Bacillus thuringiensis (Bt) are stored in the bacterial cells in crystalline form. Here we describe the structure determination of the Cry3A toxin found naturally crystallized within Bt cells. When whole Bt cells were streamed into an X-ray free-electron laser beam we found that scattering from other cell components did not obscure diffraction from the crystals. The resolution limits of the best diffraction images collected from cells were the same as from isolated crystals. The integrity of the cells at the moment of diffraction is unclear; however, given the short time (∼ 5 µs) between exiting the injector to intersecting with the X-ray beam, our result is a 2.9-Å-resolution structure of a crystalline protein as it exists in a living cell. The study suggests that authentic in vivo diffraction studies can produce atomic-level structural information.

Details

einblenden:
ausblenden:
Sprache(n): eng - Englisch
 Datum: 2014-07-232014-08-182014-08-182014-09-02
 Publikationsstatus: Im Druck publiziert
 Seiten: 6
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Proceedings of the National Academy of Sciences of the United States of America
  Andere : Proc. Natl. Acad. Sci. U. S. A.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: National Academy of Sciences
Seiten: - Band / Heft: 111 (35) Artikelnummer: - Start- / Endseite: 12769 - 12774 Identifikator: ISSN: 0027-8424
CoNE: /journals/resource/954925427230