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  Accurate Protein Complex Retrieval by Affinity Enrichment Mass Spectrometry (AE-MS) Rather than Affinity Purification Mass Spectrometry (AP-MS)

Keilhauer, E. C., Hein, M. Y., & Mann, M. (2015). Accurate Protein Complex Retrieval by Affinity Enrichment Mass Spectrometry (AE-MS) Rather than Affinity Purification Mass Spectrometry (AP-MS). MOLECULAR & CELLULAR PROTEOMICS, 14(1), 120-135. doi:10.1074/mcp.M114.041012.

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Mol Cell Proteomics-2015-Keilhauer-120-35.pdf (beliebiger Volltext), 2MB
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 Urheber:
Keilhauer, Eva C.1, Autor           
Hein, Marco Y.1, Autor           
Mann, Matthias1, Autor           
Affiliations:
1Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Schlagwörter: NUCLEAR-PORE COMPLEX, SACCHAROMYCES-CEREVISIAE, QUANTITATIVE PROTEOMICS, MACROMOLECULAR COMPLEXES, BAC TRANSGENEOMICS, YEAST, QUANTIFICATION, IDENTIFICATION, MIXTURES, SYSTEMChemistry;
 Zusammenfassung: Protein-protein interactions are fundamental to the understanding of biological processes. Affinity purification coupled to mass spectrometry (AP-MS) is one of the most promising methods for their investigation. Previously, complexes were purified as much as possible, frequently followed by identification of individual gel bands. However, todays mass spectrometers are highly sensitive, and powerful quantitative proteomics strategies are available to distinguish true interactors from background binders. Here we describe a high performance affinity enrichment-mass spectrometry method for investigating protein-protein interactions, in which no attempt at purifying complexes to homogeneity is made. Instead, we developed analysis methods that take advantage of specific enrichment of interactors in the context of a large amount of unspecific background binders. We perform single-step affinity enrichment of endogenously expressed GFP-tagged proteins and their interactors in budding yeast, followed by single-run, intensity-based label-free quantitative LC-MS/MS analysis. Each pull-down contains around 2000 background binders, which are reinterpreted from troubling contaminants to crucial elements in a novel data analysis strategy. First the background serves for accurate normalization. Second, interacting proteins are not identified by comparison to a single untagged control strain, but instead to the other tagged strains. Third, potential interactors are further validated by their intensity profiles across all samples. We demonstrate the power of our AE-MS method using several well-known and challenging yeast complexes of various abundances. AE-MS is not only highly efficient and robust, but also cost effective, broadly applicable, and can be performed in any laboratory with access to high-resolution mass spectrometers.

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Sprache(n): eng - English
 Datum: 2014-112015-01
 Publikationsstatus: Erschienen
 Seiten: 16
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: ISI: 000347155500010
DOI: 10.1074/mcp.M114.041012
 Art des Abschluß: -

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Titel: MOLECULAR & CELLULAR PROTEOMICS
Genre der Quelle: Zeitschrift
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Ort, Verlag, Ausgabe: 9650 ROCKVILLE PIKE, BETHESDA, MD 20814-3996 USA : AMER SOC BIOCHEMISTRY MOLECULAR BIOLOGY INC
Seiten: - Band / Heft: 14 (1) Artikelnummer: - Start- / Endseite: 120 - 135 Identifikator: ISSN: 1535-9476