Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  Specific 13C labeling of leucine, valine and isoleucine methyl groups for unambiguous detection of long-range restraints in protein solid-state NMR studies.

Fasshuber, H. K., Demers, J. P., Chevelkov, V., Giller, K., Becker, S., & Lange, A. (2015). Specific 13C labeling of leucine, valine and isoleucine methyl groups for unambiguous detection of long-range restraints in protein solid-state NMR studies. Journal of Magnetic Resonance, 252, 10-19. doi:10.1016/j.jmr.2014.12.013.

Item is

Dateien

einblenden: Dateien
ausblenden: Dateien
:
2087019.pdf (Verlagsversion), 3MB
Name:
2087019.pdf
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/pdf / [MD5]
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
:
2087019_Suppl.docx (Ergänzendes Material), 883KB
Name:
2087019_Suppl.docx
Beschreibung:
-
OA-Status:
Sichtbarkeit:
Öffentlich
MIME-Typ / Prüfsumme:
application/vnd.openxmlformats-officedocument.wordprocessingml.document / [MD5]
Technische Metadaten:
Copyright Datum:
-
Copyright Info:
-
Lizenz:
-

Urheber

einblenden:
ausblenden:
 Urheber:
Fasshuber, H. K.1, Autor           
Demers, J. P.1, Autor           
Chevelkov, V.1, Autor           
Giller, K.2, Autor           
Becker, S.2, Autor           
Lange, A.2, Autor           
Affiliations:
1Research Group of Solid-State NMR, MPI for biophysical chemistry, Max Planck Society, ou_persistent35              
2Department of NMR-Based Structural Biology, MPI for biophysical chemistry, Max Planck Society, ou_578567              

Inhalt

einblenden:
ausblenden:
Schlagwörter: (13)C methyl labeling; Distance restraints; Dynamics; Nuclear Magnetic Resonance; Protein structure; Proton detection; Solid-state NMR; α-Ketobutyrate; α-Ketoisovalerate
 Zusammenfassung: Here we present an isotopic labeling strategy to easily obtain unambiguous long-range distance restraints in protein solid-state NMR studies. The method is based on the inclusion of two biosynthetic precursors in the bacterial growth medium, α-ketoisovalerate and α-ketobutyrate, leading to the production of leucine, valine and isoleucine residues that are exclusively 13C labeled on methyl groups. The resulting spectral simplification facilitates the collection of distance restraints, the verification of carbon chemical shift assignments and the measurement of methyl group dynamics. This approach is demonstrated on the type-three secretion system needle of Shigella flexneri, where 49 methyl-methyl and methyl-nitrogen distance restraints including 10 unambiguous long-range distance restraints could be collected. By combining this labeling scheme with ultra-fast MAS and proton detection, the assignment of methyl proton chemical shifts was achieved.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2015-01-062015-03
 Publikationsstatus: Erschienen
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1016/j.jmr.2014.12.013
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Journal of Magnetic Resonance
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: -
Seiten: - Band / Heft: 252 Artikelnummer: - Start- / Endseite: 10 - 19 Identifikator: -