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  Drosophila gene tao-1 encodes proteins with and without a Ste20 kinase domain that affect cytoskeletal architecture and cell migration differently.

Pflanz, R., Voigt, A., Yakulov, T., & Jäckle, H. (2015). Drosophila gene tao-1 encodes proteins with and without a Ste20 kinase domain that affect cytoskeletal architecture and cell migration differently. Open Biology, 5(1): 140161. doi:10.1098/rsob.140161.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-B429-1 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-2074-C
Genre: Journal Article

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Pflanz, R.1, Author              
Voigt, A., Author
Yakulov, T., Author
Jäckle, H.1, Author              
Affiliations:
1Department of Molecular Developmental Biology, MPI for biophysical chemistry, Max Planck Society, ou_578590              

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Free keywords: Ste20 kinase; cytoskeletal architecture; ectopic pole cell induction; germline cell migration
 Abstract: Tao-1, the single representative of the Sterile 20 kinase subfamily in Drosophila, is best known for destabilizing microtubules at the actin-rich cortex, regulating the cytoskeletal architecture of cells. More recently, Tao-1 was shown to act in the Salvador-Warts-Hippo pathway by phosphorylating Hippo, regulating cell growth as well as cell polarity. Here, we show that tao-1 encodes two proteins, one with the Sterile 20 kinase domain (Tao-L) and one without it (Tao-S), and that they act in an antagonistic manner. Tao-L expression causes lamellipodia-like cell protrusions, whereas Tao-S expression results in filopodia-like structures that make cells stick to the surface they attach to. Ectopic Tao-1 expression in the anterior region of Drosophila embryos results in pole cell formation as normally observed at the posterior end. Tao-S expression causes primordial germ cells (PGCs) to adhere to the inner wall of the gut primordia and prevents proper transepithelial migration to the gonads. Conversely, RNAi knockdowns of Tao-1 cause disordered migration of PGCs out of the gut epithelium, their dispersal within the embryo and cell death. The results reveal a novel function of Tao-1 in cell migration, which is based on antagonistic activities of two proteins encoded by a single gene.

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Language(s): eng - English
 Dates: 2015-01-14
 Publication Status: Published online
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1098/rsob.140161
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Title: Open Biology
Source Genre: Journal
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Pages: 11 Volume / Issue: 5 (1) Sequence Number: 140161 Start / End Page: - Identifier: -