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  The photocycle and ultrafast vibrational dynamics of bacteriorhodopsin in lipid nanodiscs

Johnson, P. J. M., Halpin, A., Morizumi, T., Brown, L. S., Prokhorenko, V., Ernst, O. P., et al. (2014). The photocycle and ultrafast vibrational dynamics of bacteriorhodopsin in lipid nanodiscs. Physical Chemistry Chemical Physics, 16(39), 21310-21320. doi:10.1039/C4CP01826E.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-BE82-7 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0028-1972-7
Genre: Journal Article

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https://dx.doi.org/10.1039/C4CP01826E (Publisher version)
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 Creators:
Johnson, Philip J. M.1, Author
Halpin, Alexei1, Author
Morizumi, Takefumi2, Author
Brown, Leonid S.3, Author
Prokhorenko, Valentyn4, Author              
Ernst, Oliver P.2, 5, Author
Miller, R. J. Dwayne1, 4, Author              
Affiliations:
1Institute for Optical Sciences & Departments of Chemistry & Physics, University of Toronto, 80 St. George Street, Toronto, Canada , ou_persistent22              
2Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, Canada , ou_persistent22              
3Department of Physics and Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, Canada , ou_persistent22              
4Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
5Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, Canada , ou_persistent22              

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 Abstract: The photocycle and vibrational dynamics of bacteriorhodopsin in a lipid nanodisc microenvironment have been studied by steady-state and time-resolved spectroscopies. Linear absorption and circular dichroism indicate that the nanodiscs do not perturb the structure of the retinal binding pocket, while transient absorption and flash photolysis measurements show that the photocycle which underlies proton pumping is unchanged from that in the native purple membranes. Vibrational dynamics during the initial photointermediate formation are subsequently studied by ultrafast broadband transient absorption spectroscopy, where the low scattering afforded by the lipid nanodisc microenvironment allows for unambiguous assignment of ground and excited state nuclear dynamics through Fourier filtering of frequency regions of interest and subsequent time domain analysis of the retrieved vibrational dynamics. Canonical ground state oscillations corresponding to high frequency ethylenic and C-C stretches, methyl rocks, and hydrogen out-of-plane wags are retrieved, while large amplitude, short dephasing time vibrations are recovered predominantly in the frequency region associated with out-of-plane dynamics and low frequency torsional modes implicated in isomerization.

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Language(s): eng - English
 Dates: 2014-04-282014-08-102014-08-142014-10-21
 Publication Status: Published in print
 Pages: 11
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1039/C4CP01826E
 Degree: -

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Title: Physical Chemistry Chemical Physics
  Abbreviation : Phys. Chem. Chem. Phys.
Source Genre: Journal
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Publ. Info: Cambridge, England : Royal Society of Chemistry
Pages: - Volume / Issue: 16 (39) Sequence Number: - Start / End Page: 21310 - 21320 Identifier: ISSN: 1463-9076
CoNE: /journals/resource/954925272413_1