Deutsch
 
Hilfe Datenschutzhinweis Impressum
  DetailsucheBrowse

Datensatz

DATENSATZ AKTIONENEXPORT
  The photocycle and ultrafast vibrational dynamics of bacteriorhodopsin in lipid nanodiscs

Johnson, P. J. M., Halpin, A., Morizumi, T., Brown, L. S., Prokhorenko, V., Ernst, O. P., et al. (2014). The photocycle and ultrafast vibrational dynamics of bacteriorhodopsin in lipid nanodiscs. Physical Chemistry Chemical Physics, 16(39), 21310-21320. doi:10.1039/C4CP01826E.

Item is

Externe Referenzen

einblenden:
ausblenden:
externe Referenz:
https://dx.doi.org/10.1039/C4CP01826E (Verlagsversion)
Beschreibung:
-
OA-Status:

Urheber

einblenden:
ausblenden:
 Urheber:
Johnson, Philip J. M.1, Autor
Halpin, Alexei1, Autor
Morizumi, Takefumi2, Autor
Brown, Leonid S.3, Autor
Prokhorenko, Valentyn4, Autor           
Ernst, Oliver P.2, 5, Autor
Miller, R. J. Dwayne1, 4, Autor           
Affiliations:
1Institute for Optical Sciences & Departments of Chemistry & Physics, University of Toronto, 80 St. George Street, Toronto, Canada , ou_persistent22              
2Department of Biochemistry, University of Toronto, 1 King's College Circle, Toronto, Canada , ou_persistent22              
3Department of Physics and Biophysics Interdepartmental Group, University of Guelph, 50 Stone Road East, Guelph, Canada , ou_persistent22              
4Miller Group, Atomically Resolved Dynamics Department, Max Planck Institute for the Structure and Dynamics of Matter, Max Planck Society, ou_1938288              
5Department of Molecular Genetics, University of Toronto, 1 King's College Circle, Toronto, Canada , ou_persistent22              

Inhalt

einblenden:
ausblenden:
Schlagwörter: -
 Zusammenfassung: The photocycle and vibrational dynamics of bacteriorhodopsin in a lipid nanodisc microenvironment have been studied by steady-state and time-resolved spectroscopies. Linear absorption and circular dichroism indicate that the nanodiscs do not perturb the structure of the retinal binding pocket, while transient absorption and flash photolysis measurements show that the photocycle which underlies proton pumping is unchanged from that in the native purple membranes. Vibrational dynamics during the initial photointermediate formation are subsequently studied by ultrafast broadband transient absorption spectroscopy, where the low scattering afforded by the lipid nanodisc microenvironment allows for unambiguous assignment of ground and excited state nuclear dynamics through Fourier filtering of frequency regions of interest and subsequent time domain analysis of the retrieved vibrational dynamics. Canonical ground state oscillations corresponding to high frequency ethylenic and C-C stretches, methyl rocks, and hydrogen out-of-plane wags are retrieved, while large amplitude, short dephasing time vibrations are recovered predominantly in the frequency region associated with out-of-plane dynamics and low frequency torsional modes implicated in isomerization.

Details

einblenden:
ausblenden:
Sprache(n): eng - English
 Datum: 2014-04-282014-08-102014-08-142014-10-21
 Publikationsstatus: Erschienen
 Seiten: 11
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1039/C4CP01826E
 Art des Abschluß: -

Veranstaltung

einblenden:

Entscheidung

einblenden:

Projektinformation

einblenden:

Quelle 1

einblenden:
ausblenden:
Titel: Physical Chemistry Chemical Physics
  Kurztitel : Phys. Chem. Chem. Phys.
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: Cambridge, England : Royal Society of Chemistry
Seiten: - Band / Heft: 16 (39) Artikelnummer: - Start- / Endseite: 21310 - 21320 Identifikator: ISSN: 1463-9076
CoNE: https://pure.mpg.de/cone/journals/resource/954925272413_1