English
 
User Manual Privacy Policy Disclaimer Contact us
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Vascular importance of themiR-212/132 cluster.

Kumarswamy, R., Volkmann, I., Beermann, J., Napp, L. C., Jabs, O., Bhayadia, R., et al. (2014). Vascular importance of themiR-212/132 cluster. European Heart Journal, 35(45), 3224-3231. doi:10.1093/eurheartj/ehu344.

Item is

Basic

show hide
Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-B4DD-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0027-CC2D-4
Genre: Journal Article

Files

show Files
hide Files
:
2087276.pdf (Publisher version), 1015KB
 
File Permalink:
-
Name:
2087276.pdf
Description:
-
Visibility:
Restricted (Max Planck Institute for Biophysical Chemistry (Karl Friedrich Bonhoeffer Institute), Göttingen; )
MIME-Type / Checksum:
application/pdf
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-
:
2087276_Suppl.DC1 (Supplementary material), 37KB
Name:
2087276_Suppl.DC1
Description:
-
Visibility:
Public
MIME-Type / Checksum:
text/html / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
-
License:
-

Locators

show

Creators

show
hide
 Creators:
Kumarswamy, R., Author
Volkmann, I., Author
Beermann, J., Author
Napp, L. C., Author
Jabs, O., Author
Bhayadia, R., Author
Melk, A., Author
Ucar, A.1, Author              
Chowdhury, K.2, Author              
Lorenzen, J. M., Author
Gupta, S. K., Author
Batkai, S., Author
Thum, T., Author
Affiliations:
1Research Group of Molecular Developmental Neurobiology, MPI for biophysical chemistry, Max Planck Society, ou_578587              
2Facility of Microarray Analyses, MPI for biophysical chemistry, Max Planck Society, ou_578589              

Content

show
hide
Free keywords: Angiogenesis; MicroRNAs; Endothelial cells; Sirtuins; Vascular function
 Abstract: Rationale Many processes in endothelial cells including angiogenic responses are regulated by microRNAs. However, there is limited information available about their complex cross-talk in regulating certain endothelial functions. Aim The objective of this study is to identify endothelial functions of the pro-hypertrophic miR-212/132 cluster and its crosstalk with other microRNAs during development and disease. Methods and results We here show that anti-angiogenic stimulation by transforming growth factor-beta activates the microRNA-212/132 cluster by derepression of their transcriptional co-activator cAMP response element-binding protein (CREB)-binding protein (CBP) which is a novel target of a previously identified pro-angiogenic miRNA miR-30a-3p in endothelial cells. Surprisingly, despite having the same seed-sequence, miR-212 and miR-132 exerted differential effects on endothelial transcriptome regulation and cellular functions with stronger endothelial inhibitory effects caused by miR-212. These differences could be attributed to additional auxiliary binding of miR-212 to its targets. In vivo, deletion of the miR-212/132 cluster increased endothelial vasodilatory function, improved angiogenic responses during postnatal development and in adult mice. Conclusion Our results identify (i) a novel miRNA-cross-talk involving miR-30a-3p and miR-212, which led to suppression of important endothelial genes such as GAB1 and SIRT1 finally culminating in impaired endothelial function; and (ii) microRNAs may have different biological roles despite having the same seed sequence.

Details

show
hide
Language(s): eng - English
 Dates: 2014-09-122014-12-01
 Publication Status: Published in print
 Pages: -
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1093/eurheartj/ehu344
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: European Heart Journal
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: -
Pages: - Volume / Issue: 35 (45) Sequence Number: - Start / End Page: 3224 - 3231 Identifier: -