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  The exocytotic event in chromaffin cells revealed by patch amperometry

Albillos, A. C., Dernick, G., Horstmann, H., Almers, W., Alvarez de Toledo, G., & Lindau, M. (1997). The exocytotic event in chromaffin cells revealed by patch amperometry. Nature, 389(6650), 509-512. doi:10.1038/39081.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-B769-E Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-7285-D
Genre: Journal Article
Alternative Title : The exocytotic event in chromaffin cells revealed by patch amperometry

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Nature_389_1997_509.pdf (Any fulltext), 346KB
 
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 Creators:
Albillos, Almudena C.1, Author              
Dernick, Gregor2, Author              
Horstmann, Heinz1, Author              
Almers, Wolfhard1, Author              
Alvarez de Toledo, G., Author
Lindau, Manfred1, Author              
Affiliations:
1Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              
2Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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 Abstract: In mast cells and granulocytes, exocytosis starts with the formation of a fusion pore. It has been suggested that neurotransmitters may be released through such a narrow pore without full fusion. However, owing to the small size of the secretory vesicles containing neurotransmitter, the properties of the fusion pore formed during Ca2+-dependent exocytosis and its role in transmitter release are still unknown. Here we investigate exocytosis of individual chromaffin granules by using cell-attached capacitance measurements combined with electrochemical detection of catecholamines, achieved by inserting a carbon-fibre electrode into the patch pipette. This allows the simultaneous determination of the opening of individual fusion pores and of the kinetics of catecholamine release from the same vesicle. We found that the fusion-pore diameter stays at <3 nm for a variable period, which can last for several seconds, before it expands. Transmitter is released much faster through this pore than in mast cells, generating a 'foot' signal which precedes the amperometric spike. Occasionally, the narrow pore forms only transiently and does not expand, allowing complete transmitter release without full fusion of the vesicle with the plasma membrane

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Language(s): eng - English
 Dates: 1997-06-231997-07-241997-10-02
 Publication Status: Published in print
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 Rev. Type: Peer
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Title: Nature
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 389 (6650) Sequence Number: - Start / End Page: 509 - 512 Identifier: ISSN: 0028-0836
CoNE: https://pure.mpg.de/cone/journals/resource/954925427238