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  Ca2+-triggered peptide secretion in single cells imaged with green fluorescent protein and evanescent-wave microscopy

Lang, T., Wacker-Schröder, I., Steyer, J. A., Kaether, C., Wunderlich, I., Soldati, T., et al. (1997). Ca2+-triggered peptide secretion in single cells imaged with green fluorescent protein and evanescent-wave microscopy. Neuron, 18(6), 857-863. doi:10.1016/S0896-6273(00)80325-6.

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Genre: Journal Article
Alternative Title : Ca2+-triggered peptide secretion in single cells imaged with green fluorescent protein and evanescent-wave microscopy

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Neuron_18_1997_857.pdf (Any fulltext), 378KB
 
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 Creators:
Lang, Thorsten1, Author              
Wacker-Schröder, Irene2, Author              
Steyer, Jürgen A.1, Author              
Kaether, Christoph, Author
Wunderlich, Ilse3, Author              
Soldati, Thierry3, Author              
Gerdes, Hans Herman, Author
Almers, Wolfhard1, Author              
Affiliations:
1Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              
2Emeritus Group Biophysics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497712              
3Department of Biomedical Optics, Max Planck Institute for Medical Research, Max Planck Society, ou_1497699              

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 Abstract: Green fluorescent protein fused to human chromogranin B or neuropeptide y was expressed in PC12 cells and caused bright, punctate fluorescence. The fluorescent points colocalized with the endogenous secretory granule marker dopamine β−hydroxylase. Stimulation of live PC12 cells with elevated [K+], or of permeabilized PC12 cells with Ca2+, led to Ca2+−dependent loss of fluorescence from neurites. Ca2+ stimulated secretion of both fusion proteins equally well. In living cells, single fluorescent granules were imaged by evanescent−wave fluorescence microscopy. Granules were seen to migrate; to stop, as if trapped by plasmalemmal docking sites; and then to disappear abruptly, as if through exocytosis. Evidently, GFP fused to secreted peptides is a fluorescent marker for dense−core secretory granules and may be used for time−resolved microscopy of single granules

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Language(s): eng - English
 Dates: 1997-04-071997-05-011997-06-01
 Publication Status: Published in print
 Pages: 7
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: Neuron
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 18 (6) Sequence Number: - Start / End Page: 857 - 863 Identifier: ISSN: 0896-6273
CoNE: https://pure.mpg.de/cone/journals/resource/954925560565