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  TEM preparation methods and influence of radiation damage on the beam sensitive CaCO3 shell of Emiliania huxleyi

Hoffmann, R., Wochnik, A. S., Betzler, S. B., Matich, S., Griesshaber, E., Schmahl, W. W., et al. (2014). TEM preparation methods and influence of radiation damage on the beam sensitive CaCO3 shell of Emiliania huxleyi. Micron, 62, 28-36. doi:10.1016/j.micron.2014.03.004.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-BC46-1 Version Permalink: http://hdl.handle.net/21.11116/0000-0001-6E5B-F
Genre: Journal Article

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 Creators:
Hoffmann, Ramona1, Author              
Wochnik, Angela S.2, Author              
Betzler, Sophia B.3, Author              
Matich, Sonja4, Author              
Griesshaber, Erika5, Author              
Schmahl, Wolfgang W.5, Author              
Scheu, Christina3, Author              
Affiliations:
1Department of Earth and Environmental Sciences, LMU Munich, Germany, ou_persistent22              
2Department of Chemistry, LMU Munich, Germany , ou_persistent22              
3Department of Chemistry, University of Munich (LMU), Butenandtstrasse 5-13, 81377 Munich, Germany, ou_persistent22              
4Walter-Schottky-Institut, TUM Garching, Germany, ou_persistent22              
5Department of Earth and Environmental Sciences, LMU Munich, Germany , ou_persistent22              

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Free keywords: ELECTRON-MICROSCOPY; COCCOLITHS; CO2; ELEMENTS; CALCITE; OCEAN; CAOMicroscopy; Transmission electron microscopy; Sample preparation; Electron energy-loss spectroscopy; Radiation damage; Calcium carbonate; Emiliania huxleyi;
 Abstract: The ultrastructure of biologically formed calcium carbonate crystals like the shell of Emiliania huxleyi depends on the environmental conditions such as pH value, temperature and salinity. Therefore, they can be used as indicator for climate changes. However, for this a detailed understanding of their crystal structure and chemical composition is required. High resolution methods like transmission electron microscopy can provide those information on the nanoscale, given that sufficiently thin samples can be prepared. In our study, we developed sample preparation techniques for cross-section and plan-view investigations and studied the sample stability under electron bombardment. In addition to the biological material (Emiliania huxleyi) we also prepared mineralogical samples (Iceland spar) for comparison. High resolution transmission electron microscopy imaging, electron diffraction and electron energy-loss spectroscopy studies revealed that all prepared samples are relatively stable under electron bombardment at an acceleration voltage of 300 kV when using a parallel illumination. Above an accumulated dose of similar to 10(5) e/nm(2) the material - independent whether its origin is biological or geological - transformed to poly-crystalline calcium oxide. (c) 2014 Elsevier Ltd. All rights reserved.

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Language(s): eng - English
 Dates: 2014-07
 Publication Status: Published in print
 Pages: 9
 Publishing info: -
 Table of Contents: -
 Rev. Method: Peer
 Degree: -

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Title: Micron
  Abbreviation : Micron
Source Genre: Journal
 Creator(s):
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Publ. Info: Oxford : Pergamon
Pages: - Volume / Issue: 62 Sequence Number: - Start / End Page: 28 - 36 Identifier: ISSN: 0968-4328
CoNE: https://pure.mpg.de/cone/journals/resource/954928585911