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  Local Ca2+ release from internal stores controls exocytosis in pituitary gonadotrophs

Tse, F. W., Tse, A., Hille, B., Horstmann, H., & Almers, W. (1997). Local Ca2+ release from internal stores controls exocytosis in pituitary gonadotrophs. Neuron, 18(1), 121-132. doi:10.1016/S0896-6273(01)80051-9.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-BC38-3 Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002B-7287-9
Genre: Journal Article
Alternative Title : Local Ca2+ release from internal stores controls exocytosis in pituitary gonadotrophs

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Neuron_18_1997_121.pdf (Any fulltext), 708KB
 
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 Creators:
Tse, Frederick W., Author
Tse, Amy, Author
Hille, Bertil, Author
Horstmann, Heinz1, Author              
Almers, Wolfhard1, Author              
Affiliations:
1Department of Molecular Cell Research, Max Planck Institute for Medical Research, Max Planck Society, ou_1497703              

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 Abstract: Exocytosis and the cell-averaged cytosolic [Ca2+], [Ca2+]i, were tracked in single gonadotrophs. Cells released 100 granules/s at 1 μM = [Ca2+]i when gonadotropin-releasing hormone (GnRH) activated IP3-mediated Ca2+ release from internal stores, but only 1 granule/s when [Ca2+]i was raised uniformly to 1 μM by other means. Strong exocytosis was then seen only at higher [Ca2+]i (half-maximal at 16 μM). Parallel second messengers did not contribute to GnRH-induced exocytosis, because IP3 alone was as effective as GnRH, and because even GnRH failed to trigger rapid exocytosis when the [Ca2+]i rise was blunted by EGTA. When [Ca2+]i was released from stores, exocytosis depended on [Ca2+]i rising rapidly, as if governed by Ca2+ flux into the cytosol. We suggest that IP3 releases Ca2+ selectively from subsurface cisternae, raising [Ca2+] near exocytic sites 5-fold above the cell average

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Language(s): eng - English
 Dates: 1996-10-251996-11-141997-01
 Publication Status: Published in print
 Pages: 12
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
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Title: Neuron
Source Genre: Journal
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Publ. Info: Cambridge, Mass. : Cell Press
Pages: - Volume / Issue: 18 (1) Sequence Number: - Start / End Page: 121 - 132 Identifier: ISSN: 0896-6273
CoNE: https://pure.mpg.de/cone/journals/resource/954925560565