English
 
Help Privacy Policy Disclaimer
  Advanced SearchBrowse

Item

ITEM ACTIONSEXPORT
  Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography

Engel, B. D., Schaffer, M., Cuellar, L. K., Villa, E., Plitzko, J. M., & Baumeister, W. (2015). Native architecture of the Chlamydomonas chloroplast revealed by in situ cryo-electron tomography. ELIFE, 4: e04889. doi:10.7554/eLife.04889.

Item is

Files

show Files
hide Files
:
elife04889.pdf (Any fulltext), 13MB
Name:
elife04889.pdf
Description:
-
Visibility:
Public
MIME-Type / Checksum:
application/pdf / [MD5]
Technical Metadata:
Copyright Date:
-
Copyright Info:
open access article
License:
-

Locators

show

Creators

show
hide
 Creators:
Engel, Benjamin D.1, Author              
Schaffer, Miroslava1, Author              
Cuellar, Luis Kuhn1, Author              
Villa, Elizabeth1, Author              
Plitzko, Jürgen M.1, Author              
Baumeister, Wolfgang1, Author              
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

Content

show
hide
Free keywords: ELECTRON TOMOGRAPHY; THYLAKOID MEMBRANES; CARBONIC-ANHYDRASE; VITREOUS SECTIONS; PHOTOSYSTEM-II; RIBULOSE-1,5-BISPHOSPHATE CARBOXYLASE; CO2-CONCENTRATING MECHANISMS; 3-DIMENSIONAL ARCHITECTURE; CONCENTRATING MECHANISM; REINHARDTII MUTANTS
 Abstract: Chloroplast function is orchestrated by the organelle's intricate architecture. By combining cryo-focused ion beam milling of vitreous Chlamydomonas cells with cryo-electron tomography, we acquired three-dimensional structures of the chloroplast in its native state within the cell. Chloroplast envelope inner membrane invaginations were frequently found in close association with thylakoid tips, and the tips of multiple thylakoid stacks converged at dynamic sites on the chloroplast envelope, implicating lipid transport in thylakoid biogenesis. Subtomogram averaging and nearest neighbor analysis revealed that RuBisCO complexes were hexagonally packed within the pyrenoid, with similar to 15 nm between their centers. Thylakoid stacks and the pyrenoid were connected by cylindrical pyrenoid tubules, physically bridging the sites of light-dependent photosynthesis and light-independent carbon fixation. Multiple parallel minitubules were bundled within each pyrenoid tubule, possibly serving as conduits for the targeted one-dimensional diffusion of small molecules such as ATP and sugars between the chloroplast stroma and the pyrenoid matrix.

Details

show
hide
Language(s): eng - English
 Dates: 2015
 Publication Status: Published in print
 Pages: 29
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000347917200002
DOI: 10.7554/eLife.04889
 Degree: -

Event

show

Legal Case

show

Project information

show

Source 1

show
hide
Title: ELIFE
Source Genre: Journal
 Creator(s):
Affiliations:
Publ. Info: SHERATON HOUSE, CASTLE PARK, CAMBRIDGE, CB3 0AX, ENGLAND : ELIFE SCIENCES PUBLICATIONS LTD
Pages: - Volume / Issue: 4 Sequence Number: e04889 Start / End Page: - Identifier: ISSN: 2050-084X