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  Dual channel RESOLFT nanoscopy by using fluorescent state kinetics.

Testa, I., D´Este, E., Urban, N. T., Balzarotti, F., & Hell, S. W. (2015). Dual channel RESOLFT nanoscopy by using fluorescent state kinetics. Nano Letters, 15(1), 103-106. doi:10.1021/nl503058k.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0024-CEA3-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-27F8-6
Genre: Journal Article

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http://pubs.acs.org/doi/ipdf/10.1021/nl503058k (Publisher version)
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 Creators:
Testa, I.1, Author              
D´Este, E.1, Author              
Urban, N. T.1, Author              
Balzarotti, F.1, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for biophysical chemistry, Max Planck Society, ou_578627              

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Free keywords: Photoswitching; nanoscopy; lifetime; low intensities
 Abstract: We show that RESOLFT fluorescence nanoscopy, a low light level scanning superresolution technique employing reversibly switchable fluorescent proteins (rsFPs), is capable of dual-channel live-cell imaging that is virtually free of chromatic errors and temporal offsets. This is accomplished using rsEGFP and Dronpa, two rsFPs having similar spectra but different kinetics of switching and fluorescence emission. Our approach is demonstrated by imaging protein distributions and dynamics in living neurons and neuronal tissues.

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Language(s): eng - English
 Dates: 2014-11-252015-01-14
 Publication Status: Published in print
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1021/nl503058k
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Title: Nano Letters
Source Genre: Journal
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Pages: - Volume / Issue: 15 (1) Sequence Number: - Start / End Page: 103 - 106 Identifier: -