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  Targeting cells with single vectors using multiple-feature Boolean logic

Fenno, L. E., Mattis, J., Ramakrishnan, C., Hyun, M., Lee, S. Y., He, M., et al. (2014). Targeting cells with single vectors using multiple-feature Boolean logic. Nature Methods, 11, 763-772. doi:10.1038/nmeth.2996.

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Datensatz-Permalink: https://hdl.handle.net/11858/00-001M-0000-0024-E29C-3 Versions-Permalink: https://hdl.handle.net/21.11116/0000-000C-862F-9
Genre: Zeitschriftenartikel

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externe Referenz:
https://www.nature.com/articles/nmeth.2996 (Verlagsversion)
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Keine Angabe

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 Urheber:
Fenno, L. E., Autor
Mattis, J., Autor
Ramakrishnan, C., Autor
Hyun, M., Autor
Lee, S. Y., Autor
He, M., Autor
Tucciarone, J., Autor
Selimbeyoglu, A., Autor
Berndt, A., Autor
Grosenick, L., Autor
Zalocusky, K. A., Autor
Bernstein, H., Autor
Swanson, H., Autor
Perry, C., Autor
Diester, Ilka1, Autor
Boyce, F. M., Autor
Bass, C. E., Autor
Neve, R., Autor
Huang, Z. J., Autor
Deisseroth, K., Autor
Affiliations:
1Ernst Strüngmann Institute (ESI) for Neuroscience in Cooperation with Max Planck Society, Max Planck Society, ou_2074314              

Inhalt

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Schlagwörter: Animals Bacterial Proteins/genetics Dependovirus/genetics Female Gene Targeting/*methods *Genetic Vectors HEK293 Cells Hippocampus/metabolism Humans Integrases/metabolism Interneurons/*physiology Introns Logic Luminescent Proteins/genetics Male Mice Mice, Transgenic Promoter Regions, Genetic Transgenes
 Zusammenfassung: Precisely defining the roles of specific cell types is an intriguing frontier in the study of intact biological systems and has stimulated the rapid development of genetically encoded tools for observation and control. However, targeting these tools with adequate specificity remains challenging: most cell types are best defined by the intersection of two or more features such as active promoter elements, location and connectivity. Here we have combined engineered introns with specific recombinases to achieve expression of genetically encoded tools that is conditional upon multiple cell-type features, using Boolean logical operations all governed by a single versatile vector. We used this approach to target intersectionally specified populations of inhibitory interneurons in mammalian hippocampus and neurons of the ventral tegmental area defined by both genetic and wiring properties. This flexible and modular approach may expand the application of genetically encoded interventional and observational tools for intact-systems biology.

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Sprache(n): eng - English
 Datum: 2014-06-08
 Publikationsstatus: Online veröffentlicht
 Seiten: -
 Ort, Verlag, Ausgabe: -
 Inhaltsverzeichnis: -
 Art der Begutachtung: Expertenbegutachtung
 Identifikatoren: DOI: 10.1038/nmeth.2996
 Art des Abschluß: -

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Titel: Nature Methods
  Andere : Nature Methods
Genre der Quelle: Zeitschrift
 Urheber:
Affiliations:
Ort, Verlag, Ausgabe: New York, NY : Nature Publishing Group
Seiten: - Band / Heft: 11 Artikelnummer: - Start- / Endseite: 763 - 772 Identifikator: ISSN: 1548-7091
CoNE: https://pure.mpg.de/cone/journals/resource/111088195279556