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  The interaction of Saccharomyces paradoxus with its natural competitors on oak bark

Kowallik, V., Miller, E., & Greig, D. (2015). The interaction of Saccharomyces paradoxus with its natural competitors on oak bark. Molecular Ecology, 24(7), 1596 -1610. doi:10.1111/mec.13120.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-05C2-C Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0026-A6D8-7
Genre: Journal Article

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Kowallik, Vienna1, Author              
Miller, Eric1, Author              
Greig, Duncan1, Author              
Affiliations:
1Max-Planck Research Group Experimental Evolution, Max Planck Institute for Evolutionary Biology, Max Planck Society, ou_1445640              

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Free keywords: Saccharomyces; bacteria; competition; ecology; fungi; natural history
 Abstract: The natural history of the model yeast Saccharomyces cerevisiae is poorly understood and confounded by domestication. In nature, S. cerevisiae and its undomesticated relative S. paradoxus, are usually found on the bark of oak trees, a habitat very different from wine or other human fermentations. It is unclear whether the oak trees are really the primary habitat for wild yeast, or whether this apparent association is due to biased sampling. We use culturing and high-throughput environmental sequencing to show that S. paradoxus is a very rare member of the oak bark microbial community. We find that S. paradoxus can grow well on sterile medium made from oak bark, but that its growth is strongly suppressed when the other members of the community are present. We purified a set of twelve common fungal and bacterial species from the oak bark community, and tested how each affected the growth of S. paradoxus in direct competition on oak bark medium at summer and winter temperatures, identifying both positive and negative interactions. One Pseudomonas species produces a diffusible toxin that suppresses S. paradoxus almost as effectively as either the whole set of twelve species together, or the complete community present in non-sterilized oak medium. Conversely, one of the twelve species, Mucilaginibacter sp., had the opposite effect and promoted S. paradoxus growth at low temperatures. We conclude that, in its natural oak tree habitat, S. paradoxus is a rare species whose success depends on the much more abundant microbial species surrounding it.

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Language(s): eng - English
 Dates: 2015-02-132014-12-122015-02-182015-02-232015-04
 Publication Status: Published in print
 Pages: -
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 Rev. Method: -
 Identifiers: DOI: 10.1111/mec.13120
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Title: Molecular Ecology
Source Genre: Journal
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Publ. Info: Oxford : Blackwell Science
Pages: - Volume / Issue: 24 (7) Sequence Number: - Start / End Page: 1596 - 1610 Identifier: ISSN: 0962-1083 (print)
ISSN: 1365-294X (online)
CoNE: /journals/resource/954925580119