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  STED nanoscopy reveals the ubiquity of subcortical cytoskeleton periodicity in living neurons.

D'Este, E., Kamin, D., Göttfert, F., El-Hady Hashem, A., & Hell, S. W. (2015). STED nanoscopy reveals the ubiquity of subcortical cytoskeleton periodicity in living neurons. Cell Reports, 10(8), 1246-1251. doi:10.1016/j.celrep.2015.02.007.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-05F3-D Version Permalink: http://hdl.handle.net/11858/00-001M-0000-002A-09ED-5
Genre: Journal Article

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 Creators:
D'Este, E.1, Author              
Kamin, D.1, Author              
Göttfert, F.1, Author              
El-Hady Hashem, A.1, Author              
Hell, S. W.1, Author              
Affiliations:
1Department of NanoBiophotonics, MPI for Biophysical Chemistry, Max Planck Society, ou_578627              

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 Abstract: In the axons of cultured hippocampal neurons, actin forms various structures, including bundles, patches (involved in the preservation of neuronal polarity), and a recently reported periodic ring-like structure. Nevertheless, the overlaying organization of actin in neurons and in the axon initial segment (AIS) is still unclear, due mainly to a lack of adequate imaging methods. By harnessing live-cell stimulated emission depletion (STED) nanoscopy and the fluorescent probe SiR-Actin, we show that the periodic subcortical actin structure is in fact present in both axons and dendrites. The periodic cytoskeleton organization is also found in the peripheral nervous system, specifically at the nodes of Ranvier. The actin patches in the AIS co-localize with pre-synaptic markers. Cytosolic actin organization strongly depends on the developmental stage and subcellular localization. Altogether, the results of this study reveal unique neuronal cytoskeletal features.

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Language(s): eng - English
 Dates: 2015-02-262015-03-03
 Publication Status: Published in print
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 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.celrep.2015.02.007
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Title: Cell Reports
Source Genre: Journal
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Pages: - Volume / Issue: 10 (8) Sequence Number: - Start / End Page: 1246 - 1251 Identifier: -