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  The histone deacetylase inhibitor trichostatin a promotes totipotency in the male gametophyte

Li, H., Soriano, M., Cordewener, J., Muino, J. M., Riksen, T., Fukuoka, H., et al. (2014). The histone deacetylase inhibitor trichostatin a promotes totipotency in the male gametophyte. The Plant Cell, 26(1), 195-209. doi:10.1105/tpc.113.116491.

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© 2014 by the American Society of Plant Biologists
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Li, H., Author
Soriano, M., Author
Cordewener, J., Author
Muino, J. M.1, Author              
Riksen, T., Author
Fukuoka, H., Author
Angenent, G. C., Author
Boutilier, K., Author
Affiliations:
1Dept. of Computational Molecular Biology (Head: Martin Vingron), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433547              

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Free keywords: Acetylation Arabidopsis/*cytology/drug effects/growth & development Arabidopsis Proteins/metabolism Cell Division/drug effects Cell Proliferation/drug effects Cell Wall/drug effects Histone Deacetylase Inhibitors/*pharmacology Histones/metabolism Hydroxamic Acids/*pharmacology Indoleacetic Acids/metabolism Pollen/cytology/drug effects/growth & development Signal Transduction/drug effects
 Abstract: The haploid male gametophyte, the pollen grain, is a terminally differentiated structure whose function ends at fertilization. Plant breeding and propagation widely use haploid embryo production from in vitro-cultured male gametophytes, but this technique remains poorly understood at the mechanistic level. Here, we show that histone deacetylases (HDACs) regulate the switch to haploid embryogenesis. Blocking HDAC activity with trichostatin A (TSA) in cultured male gametophytes of Brassica napus leads to a large increase in the proportion of cells that switch from pollen to embryogenic growth. Embryogenic growth is enhanced by, but not dependent on, the high-temperature stress that is normally used to induce haploid embryogenesis in B. napus. The male gametophyte of Arabidopsis thaliana, which is recalcitrant to haploid embryo development in culture, also forms embryogenic cell clusters after TSA treatment. Genetic analysis suggests that the HDAC protein HDA17 plays a role in this process. TSA treatment of male gametophytes is associated with the hyperacetylation of histones H3 and H4. We propose that the totipotency of the male gametophyte is kept in check by an HDAC-dependent mechanism and that the stress treatments used to induce haploid embryo development in culture impinge on this HDAC-dependent pathway.

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Language(s): eng - English
 Dates: 2014-01-242014-01
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1105/tpc.113.116491
ISSN: 1532-298X (Electronic)1040-4651 (Print)
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Title: The Plant Cell
  Abbreviation : Plant C
Source Genre: Journal
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Publ. Info: Rockville : American Society of Plant Physiologists
Pages: - Volume / Issue: 26 (1) Sequence Number: - Start / End Page: 195 - 209 Identifier: Other: 1532-298X
CoNE: https://pure.mpg.de/cone/journals/resource/1532-298X