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  Neurofibromin inactivation impairs osteocyte development in Nf1Prx1 and Nf1Col1 mouse models

Kühnisch, J., Seto, J., Lange, C., Stumpp, S., Kobus, K., Grohmann, J., et al. (2014). Neurofibromin inactivation impairs osteocyte development in Nf1Prx1 and Nf1Col1 mouse models. Bone, 66, 155-162. doi:10.1016/j.bone.2014.06.012.

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Item Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-B39D-A Version Permalink: http://hdl.handle.net/11858/00-001M-0000-0025-B39E-8
Genre: Journal Article

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© 2014 Elsevier B.V.
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 Creators:
Kühnisch, Jirko1, Author
Seto, Jong, Author
Lange, Claudia, Author
Stumpp, Sabine, Author
Kobus, Karolina1, Author
Grohmann, Julia1, Author
Elefteriou, Florent, Author
Fratzl, Peter, Author
Mundlos, Stefan2, Author              
Kolanczyk, Mateusz2, Author              
Affiliations:
1Max Planck Society, ou_persistent13              
2Research Group Development & Disease (Head: Stefan Mundlos), Max Planck Institute for Molecular Genetics, Max Planck Society, ou_1433557              

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 Abstract: Neurofibromin has been identified as a critical regulator of osteoblast differentiation. Osteoblast specific inactivation of neurofibromin in mice results in a high bone mass phenotype and hyperosteoidosis. Here, we show that inactivation of the Nf1 gene also impairs osteocyte development. We analyzed cortical bone tissue in two conditional mouse models, Nf1Prx1 and Nf1Col1, for morphological and molecular effects. Backscattered electron microscopy revealed significantly enlarged osteocyte lacunae in Nf1Prx1 and Nf1Col1 mice (level E2: ctrl=1.90+/-0.52%, Nf1Prx1=3.40+/-0.95%; ctrl 1.60+/-0.47%, Nf1Col1 2.46+/-0.91%). Moreover, the osteocyte lacunae appeared misshaped in Nf1Prx1 and Nf1Col1 mice as indicated by increased Feret ratios. Strongest osteocyte and dendritic network disorganization was observed in proximity of muscle attachment sites in Nf1Prx1 humeri. In contrast to control cells, Nf1Prx1 osteocytes contained abundant cytosolic vacuoles and accumulated immature organic matrix within the perilacunar space, a phenotype reminiscent of the hyperosteoidosis shown Nf1 deficient mice. Cortical bone lysates further revealed approx. twofold upregulated MAPK signalling in osteocytes of Nf1Prx1 mice. This was associated with transcriptional downregulation of collagens and genes involved in mechanical sensing in Nf1Prx1 and Nf1Col1 bone tissue. In contrast, matrix gla protein (MGP), phosphate regulating endopeptidase homolog, X-linked (PHEX), and genes involved in lipid metabolism were upregulated. In line with previously described hyperactivation of Nf1 deficient osteoblasts, systemic plasma levels of the bone formation markers osteocalcin (OCN) and procollagen typ I N-propeptide (PINP) were approx. twofold increased in Nf1Prx1 mice. Histochemical and molecular analysis ascertained that osteocytes in Nf1Prx1 cortical bone were viable and did not undergo apoptosis or autophagy. We conclude that loss of neurofibromin is not only critical for osteoblasts but also hinders normal osteocyte development. These findings expand the effect of neurofibromin onto yet another cell type where it is likely involved in the regulation of mechanical sensing, bone matrix composition and mechanical resistance of bone tissue.

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Language(s): eng - English
 Dates: 2014-06-172014-09
 Publication Status: Published in print
 Pages: -
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 Table of Contents: -
 Rev. Method: Peer
 Identifiers: DOI: 10.1016/j.bone.2014.06.012
ISSN: 1873-2763
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Title: Bone
  Other : Bone
Source Genre: Journal
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Publ. Info: New York : Elsevier
Pages: - Volume / Issue: 66 Sequence Number: - Start / End Page: 155 - 162 Identifier: ISSN: 8756-3282
CoNE: https://pure.mpg.de/cone/journals/resource/954927629281