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  Ctk1 Function Is Necessary for Full Translation Initiation Activity in Saccharomyces cerevisiae

Coordes, B., Brünger, K. M., Burger, K., Soufi, B., Horenk, J., Eick, D., et al. (2015). Ctk1 Function Is Necessary for Full Translation Initiation Activity in Saccharomyces cerevisiae. EUKARYOTIC CELL, 14(1), 86-95. doi:10.1128/EC.00106-14.

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 Creators:
Coordes, Britta1, Author
Brünger, Katharina M.1, Author
Burger, Kaspar1, Author
Soufi, Boumediene2, Author              
Horenk, Juliane1, Author
Eick, Dirk1, Author
Olsen, Jesper V.2, Author              
Strässer, Katja1, Author
Affiliations:
1external, ou_persistent22              
2Mann, Matthias / Proteomics and Signal Transduction, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565159              

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Free keywords: RNA-POLYMERASE-II; INTERNAL RIBOSOME ENTRY; CTD KINASE-I; YEAST; TRANSCRIPTION; LOCALIZATION; EUKARYOTES; ELONGATION; EXPRESSION; MECHANISM
 Abstract: Translation is a fundamental and highly regulated cellular process. Previously, we reported that the kinase and transcription elongation factor Ctk1 increases fidelity during translation elongation in Saccharomyces cerevisiae. Here, we show that loss of Ctk1 function also affects the initiation step of translation. Translation active extracts from Ctk1-depleted cells show impaired translation activity of capped mRNA, but not mRNA reporters containing the cricket paralysis virus (CrPV) internal ribosome entry site (IRES). Furthermore, the formation of 80S initiation complexes is decreased, which is probably due to reduced subunit joining. In addition, we determined the changes in the phosphorylation pattern of a ribosome enriched fraction after depletion of Ctk1. Thus, we provide a catalogue of phosphoproteomic changes dependent on Ctk1. Taken together, our data suggest a stimulatory function of Ctk1 in 80S formation during translation initiation.

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Language(s): eng - English
 Dates: 2015-01
 Publication Status: Published in print
 Pages: 10
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000348924000009
DOI: 10.1128/EC.00106-14
 Degree: -

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Title: EUKARYOTIC CELL
Source Genre: Journal
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Publ. Info: 1752 N ST NW, WASHINGTON, DC 20036-2904 USA : AMER SOC MICROBIOLOGY
Pages: - Volume / Issue: 14 (1) Sequence Number: - Start / End Page: 86 - 95 Identifier: ISSN: 1535-9778