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  Architecture and ssDNA interaction of the Timeless-Tipin-RPA complex

Witosch, J., Wolf, E., & Mizuno, N. (2014). Architecture and ssDNA interaction of the Timeless-Tipin-RPA complex. NUCLEIC ACIDS RESEARCH, 42(20), 12912-12927. doi:10.1093/nar/gku960.

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 Creators:
Witosch, Justine1, Author              
Wolf, Eva2, Author              
Mizuno, Naoko3, Author              
Affiliations:
1Conti, Elena / Structural Cell Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565144              
2Former Research Groups, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565145              
3Mizuno, Naoko / Cellular and Membrane Trafficking, Max Planck Institute of Biochemistry, Max Planck Society, ou_1688137              

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Free keywords: REPLICATION PROTEIN-A; SINGLE-STRANDED-DNA; 70 KDA SUBUNIT; CRYO-EM; ELECTRON-MICROSCOPY; BINDING DOMAINS; CHECKPOINT; FORK; TIM; XPA
 Abstract: The Timeless-Tipin (Tim-Tipin) complex, also referred to as the fork protection complex, is involved in coordination of DNA replication. Tim-Tipin is suggested to be recruited to replication forks via Replication Protein A (RPA) but details of the interaction are unknown. Here, using cryo-EM and biochemical methods, we characterized complex formation of Tim-Tipin, RPA and single-stranded DNA (ssDNA). Tim-Tipin and RPA form a 258 kDa complex with a 1:1:1 stoichiometry. The cryo-EM 3D reconstruction revealed a globular architecture of the Tim-Tipin-RPA complex with a ring-like and a U-shaped domain covered by a RPA lid. Interestingly, RPA in the complex adopts a horse shoe-like shape resembling its conformation in the presence of long ssDNA (>30 nucleotides). Furthermore, the recruitment of the TimTipin- RPA complex to ssDNA is modulated by the RPA conformation and requires RPA to be in the more compact 30 nt ssDNA binding mode. The dynamic formation and disruption of the Tim-Tipin-RPA-ssDNA complex implicates the RPA-based recruitment of Tim-Tipin to the replication fork.

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Language(s): eng - English
 Dates: 2014
 Publication Status: Published in print
 Pages: 16
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000347693200051
DOI: 10.1093/nar/gku960
 Degree: -

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Title: NUCLEIC ACIDS RESEARCH
Source Genre: Journal
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Publ. Info: GREAT CLARENDON ST, OXFORD OX2 6DP, ENGLAND : OXFORD UNIV PRESS
Pages: - Volume / Issue: 42 (20) Sequence Number: - Start / End Page: 12912 - 12927 Identifier: ISSN: 0305-1048