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  Putting a finishing touch on GEC's

Rose, T., Goltstein, P., Portugues, R., & Griesbeck, O. (2014). Putting a finishing touch on GEC's. Frontiers in Molecular Neuroscience, 7: 88. doi:10.3389/fnmol.2014.00088.

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 Creators:
Rose, Tobias1, Author              
Goltstein, Pieter1, Author              
Portugues, Ruben2, Author              
Griesbeck, Oliver3, Author              
Affiliations:
1Department: Synapses-Circuits-Plasticity / Bonhoeffer, MPI of Neurobiology, Max Planck Society, ou_1113545              
2Max Planck Research Group: Sensorimotor Control / Portugues, MPI of Neurobiology, Max Planck Society, ou_2054291              
3Research Group: Cellular Dynamics / Griesbeck, MPI of Neurobiology, Max Planck Society, ou_1113560              

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Free keywords: GREEN FLUORESCENT PROTEIN; ENCODED CALCIUM INDICATOR; IMAGING NEURAL ACTIVITY; IN-VIVO; NEURONAL-ACTIVITY; CA2+ INDICATORS; TROPONIN-C; CELLULAR RESOLUTION; SYNAPTIC PLASTICITY; POPULATION ACTIVITYbuffering; calcium; fluorescent protein; FRET; imaging; neuronal activity; segmentation;
 Abstract: More than a decade ago genetically encoded calcium indicators (GECIs) entered the stage as new promising tools to image calcium dynamics and neuronal activity in living tissues and designated cell types in vivo. From a variety of initial designs two have emerged as promising prototypes for further optimization: FRET (Forster Resonance Energy Transfer)based sensors and single fluorophore sensors of the GCaMP family. Recent efforts in structural analysis, engineering and screening have broken important performance thresholds in the latest generation for both classes. While these improvements have made GECIs a powerful means to perform physiology in living animals, a number of other aspects of sensor function deserve attention. These aspects include indicator linearity, toxicity and slow response kinetics. Furthermore creating high performance sensors with optically more favorable emission in red or infrared wavelengths as well as new stably or conditionally GECI-expressing animal lines are on the wish list. When the remaining issues are solved, imaging of GECIs will finally have crossed the last milestone, evolving from an initial promise into a fully matured technology.

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Language(s): eng - English
 Dates: 2014
 Publication Status: Published online
 Pages: 15
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: ISI: 000348067500001
DOI: 10.3389/fnmol.2014.00088
 Degree: -

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Title: Frontiers in Molecular Neuroscience
Source Genre: Journal
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Publ. Info: Lausanne, Switzerland : Frontiers Research Foundation
Pages: - Volume / Issue: 7 Sequence Number: 88 Start / End Page: - Identifier: ISSN: 1662-5099
CoNE: https://pure.mpg.de/cone/journals/resource/1662-5099