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  Architecture of the RNA polymerase II–Mediator core initiation complex.

Plaschka, C., Larivière, L., Wenzeck, L., Seizl, M., Hemann, M., Tegunov, D., et al. (2015). Architecture of the RNA polymerase II–Mediator core initiation complex. Nature, 518, 376-380. doi:10.1038/nature14229.

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Plaschka, C., Author
Larivière, L., Author
Wenzeck, L., Author
Seizl, M., Author
Hemann, M., Author
Tegunov, D.1, Author              
Petrotchenko, E. V., Author
Borchers, C. H., Author
Baumeister, W.1, Author              
Herzog, F., Author
Villa, E.1, Author              
Cramer, P., Author
Affiliations:
1Baumeister, Wolfgang / Molecular Structural Biology, Max Planck Institute of Biochemistry, Max Planck Society, ou_1565142              

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 Abstract: The conserved co-activator complex Mediator enables regulated transcription initiation by RNA polymerase (Pol) II. Here we reconstitute an active 15-subunit core Mediator (cMed) comprising all essential Mediator subunits from Saccharomyces cerevisiae. The cryo-electron microscopic structure of cMed bound to a core initiation complex was determined at 9.7 Å resolution. cMed binds Pol II around the Rpb4–Rpb7 stalk near the carboxy-terminal domain (CTD). The Mediator head module binds the Pol II dock and the TFIIB ribbon and stabilizes the initiation complex. The Mediator middle module extends to the Pol II foot with a ‘plank’ that may influence polymerase conformation. The Mediator subunit Med14 forms a ‘beam’ between the head and middle modules and connects to the tail module that is predicted to bind transcription activators located on upstream DNA. The Mediator ‘arm’ and ‘hook’ domains contribute to a ‘cradle’ that may position the CTD and TFIIH kinase to stimulate Pol II phosphorylation.

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Language(s): eng - English
 Dates: 2015-02-19
 Publication Status: Published in print
 Pages: 5
 Publishing info: -
 Table of Contents: -
 Rev. Type: Peer
 Identifiers: DOI: 10.1038/nature14229
 Degree: -

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Title: Nature
Source Genre: Journal
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Publ. Info: London : Nature Publishing Group
Pages: - Volume / Issue: 518 Sequence Number: - Start / End Page: 376 - 380 Identifier: ISSN: 0028-0836
CoNE: https://pure.mpg.de/cone/journals/resource/954925427238